Arabidopsis ARGONAUTE 1 Binds Chromatin to Promote Gene Transcription in Response to Hormones and Stresses
Arabidopsis ARGONAUTE 1 Binds Chromatin to Promote Gene Transcription in Response to Hormones and Stresses
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拟南芥 ARGONAUTE 1 结合染色质促进基因转录以响应激素和应激
DOI：
10.1016/j.devcel.2017.12.002
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发表时间：
2018-02-05
影响因子：
11.8
通讯作者：
Qi, Yijun
中科院分区：
文献类型：
--
作者：
Liu, Chang;Xin, Ying;Qi, Yijun
常规的RNA干扰（RNAi）途径在转录或转录后水平抑制真核基因表达。RNAi的核心是小RNA（sRNA）和效应子Argonaute（AGO）蛋白。已知拟南芥AGO 1结合microRNA（miRNAs）并转录后抑制细胞质中的靶基因。在这里，我们报告说，AGO 1也结合到染色质的活性基因，并促进其转录。我们发现，sRNA和SWI/SNF复合物与细胞核AGO 1相关，并且是AGO 1与染色质结合所必需的。此外，我们表明，各种刺激，包括植物激素和压力，具体触发AGO 1结合刺激响应基因。最后，我们表明，AGO 1促进茉莉酸（JA）信号通路中的基因的诱导和JA反应的激活。我们的研究结果表明，通过结合和促进刺激特异性基因的表达，AGO 1可以调节不同的信号通路和相关的生物过程。
Conventional RNA interference (RNAi) pathways suppress eukaryotic gene expression at the transcriptional or post-transcriptional level. At the core of RNAi are small RNAs (sRNAs) and effector Argonaute (AGO) proteins. Arabidopsis AGO1 is known to bind microRNAs (miRNAs) and post-transcriptionally repress target genes in the cytoplasm. Here, we report that AGO1 also binds to the chromatin of active genes and promotes their transcription. We show that sRNAs and SWI/SNF complexes associate with nuclear AGO1 and are required for AGO1 binding to chromatin. Moreover, we show that various stimuli, including plant hormones and stresses, specifically trigger AGO1 binding to stimulus-responsive genes. Finally, we show that AGO1 facilitates the induction of genes in jasmonate (JA) signaling pathways and the activation of JA responses. Our findings suggest that, by binding and facilitating the expression of stimuli-specific genes, AGO1 may regulate diverse signaling pathways and associated biological processes.