DNA RECOGNITION AND CLEAVAGE BY THE ECOP15 RESTRICTION ENDONUCLEASE
DNA RECOGNITION AND CLEAVAGE BY THE ECOP15 RESTRICTION ENDONUCLEASE
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DOI：
10.1016/0022-2836(79)90372-3
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发表时间：
1979-01-01
影响因子：
5.6
通讯作者：
BICKLE, TA
中科院分区：
文献类型：
--
作者：
HADI, SM;BACHI, B;BICKLE, TA
EcoP15是由大肠杆菌P15质粒编码的一种限制性内切酶。测定了该酶在pBR322和SV-40DNA上的识别位点。该酶识别序列：.**图形**。在限制条件下，该酶将DNA 25-26个碱基对3‘’切割到这个序列上，留下单链5‘’突起2个碱基长。
EcoP15 is a restriction-modification enzyme coded by the P15 plasmid of Escherichia coli. The sites recognized by this enzyme on pBR322 plasmid and SV-40 DNA were determined. The enzyme recognizes the sequence: .**GRAPHIC**. In restriction, the enzyme cleaves the DNA 25-26 base-pairs 3'' to this sequence to leave single-stranded 5'' protrusions 2 bases long.