The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells
The Tetraspanin CD9 Affords High-Purity Capture of All Murine Hematopoietic Stem Cells
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DOI：
10.1016/j.celrep.2013.07.020
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发表时间：
2013-08-01
期刊：
影响因子：
8.8
通讯作者：
Enver, Tariq
中科院分区：
文献类型：
--
作者：
Karlsson, Goeran;Roerby, Emma;Enver, Tariq
前瞻性分离对于理解干细胞异质性的细胞和分子方面至关重要。在这里，我们确定细胞表面抗原CD9作为阳性标记，提供了一个简单的替代造血干细胞分离在高纯度。至关重要的是，CD9在不存在缺乏真实干细胞功能的污染群体的情况下提供了鼠骨髓中所有造血干细胞的捕获。使用CD9作为一种工具，以细分造血干细胞的人口，我们提供的异质性在细胞，功能和分子水平的证据。
Prospective isolation is critical for understanding the cellular and molecular aspects of stem cell heterogeneity. Here, we identify the cell surface antigen CD9 as a positive marker that provides a simple alternative for hematopoietic stem cell isolation at high purity. Crucially, CD9 affords the capture of all hematopoietic stem cells in murine bone marrow in the absence of contaminating populations that lack authentic stem cell function. Using CD9 as a tool to subdivide hematopoietic stem-cell-containing populations, we provide evidence for heterogeneity at the cellular, functional, and molecular levels.