Stimulated endothelial cell adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene.
Stimulated endothelial cell adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene.
复制标题
用膨胀聚四氟乙烯的层粘连蛋白 5 修饰刺激内皮细胞粘附和血管生成。
DOI：
10.1089/ten.2005.11.1379
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Williams,StuartK
中科院分区：
文献类型：
--
作者：
Kidd,KamehaR;DalPonte,Donny;Stone,AliceL;Hoying,JamesB;Nagle,RaymondB;Williams,StuartK
Biomedical implants often exhibit poor clinical performance due to the formation of a periimplant avascular fibrous capsule. Surface modification of synthetic materials has been evaluated to accelerate the formation of functional microcirculation in association with implants. The current study used a flow-mediated protein deposition system to modify expanded polytetrafluoroethylene (ePTFE) with a laminin-5-rich conditioned growth medium and with medium from which laminin-5 had been selectively removed. Anin vitromodel of endothelial cell adherence determined that laminin-5 modification resulted in significantly increased adhesion of human microvessel endothelial cells to ePTFE.In vivostudies evaluating the periimplant vascular response to laminin-5-treated samples indicated that absorption of laminin-5-rich conditioned medium supported accelerated neovascularization of ePTFE implants. A flow system designed to treat porous implant materials facilitates laminin-5 modification of commercially available ePTFE, resulting in increased endothelial cell adhesion in vitro and increased vascularizationin vivo.
