Protein adsorption and transport in dextran-modified ion-exchange media. I: adsorption.
Protein adsorption and transport in dextran-modified ion-exchange media. I: adsorption.
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DOI：
10.1016/j.chroma.2009.09.014
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发表时间：
2009-11-06
期刊：
影响因子：
--
通讯作者：
Lenhoff AM
中科院分区：
文献类型：
--
作者：
Bowes BD;Koku H;Czymmek KJ;Lenhoff AM
比较了传统的琼脂糖基离子交换树脂和两种葡聚糖修饰的树脂的吸附行为，使用三种蛋白质来考察蛋白质大小的影响。后者通常在低离子强度下表现出较高的静态容量，电子显微镜提供了直接的视觉证据，支持较高的静态容量是由于葡聚糖提供了更大的可用结合体积。然而，等度保留实验表明，在高离子强度下，较大的蛋白质几乎完全被排除在葡聚糖层之外，这可能导致在相关柱负载条件下的静态容量的显著损失。树脂和蛋白质性质的知识被用来估计树脂静态容量的物理极限，以便对观察到的静态容量提供有意义的解释。这些估计的结果与传统树脂的可用表面积有限的预期一致。然而，在葡聚糖修饰的介质中，当蛋白质电荷相对于树脂电荷较低时，葡聚糖层的体积似乎限制了吸附，但当蛋白质电荷相对较高时，蛋白质-树脂的电中性可能是限制的。当与蛋白质运输信息结合时，这种分析可能被证明对最大静态容量的半定量预测和操作条件的选择有用。
Adsorption behavior is compared on a traditional agarose-based ion-exchange resin and on two dextran-modified resins, using three proteins to examine the effect of protein size. The latter resins typically exhibit higher static capacities at low ionic strengths and electron microscopy provides direct visual evidence supporting the view that the higher static capacities are due to the larger available binding volume afforded by the dextran. However, isocratic retention experiments reveal that the larger proteins can be almost completely excluded from the dextran layer at high ionic strengths, potentially leading to significant losses in static capacity at relevant column loading conditions. Knowledge of resin and protein properties is used to estimate physical limits on the static capacities of the resins in order to provide a meaningful interpretation of the observed static capacities. Results of such estimates are consistent with the expectation that available surface area is limiting for traditional resins. In dextran-modified media, however, the volume of the dextran layer appears to limit adsorption when the protein charge is low relative to the resin charge, but the protein-resin electroneutrality may be limiting when the protein charge is relatively high. Such analyses may prove useful for semiquantitative prediction of maximum static capacities and selection of operating conditions when combined with protein transport information.
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DOI：
10.1098/rspa.1999.0433
发表时间：
1999-08-08
影响因子：
3.5
作者：
Bowen, WR;Cao, XW;Williams, PM
通讯作者：
Williams, PM
DOI：
10.1073/pnas.0237084100
发表时间：
2003-01-21
影响因子：
11.1
作者：
Dziennik, SR;Belcher, EB;Lenhoff, AM
通讯作者：
Lenhoff, AM
影响因子：
4.1
作者：
BOARDMAN, NK;PARTRIDGE, SM
通讯作者：
PARTRIDGE, SM
影响因子：
4.1
作者：
Dismer, Florian;Petzold, Martin;Hubbuch, Juergen
通讯作者：
Hubbuch, Juergen
影响因子：
4.1
作者：
Fernandez, MA;Carta, G
通讯作者：
Carta, G