Dissecting the in vivo metabolic potential of two human gut acetogens.
Dissecting the in vivo metabolic potential of two human gut acetogens.
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DOI：
10.1074/jbc.m110.117713
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发表时间：
2010-07-16
期刊：
影响因子：
--
通讯作者：
Gordon JI
中科院分区：
文献类型：
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作者：
Rey FE;Faith JJ;Bain J;Muehlbauer MJ;Stevens RD;Newgard CB;Gordon JI
发酵微生物群落产生氢气;通过产生乙酸盐、甲烷或硫化氢来去除氢气，调节了许多生态系统中从可用营养物中提取能量的效率。我们注意到，产乙酸的途径组分在单卵双胞胎及其母亲的肠道微生物组中比产甲烷或硫酸盐还原的组分更丰富和一致，随后分析了两种测序的人类肠道产乙酸菌Blautia hydrogenotrophica和Marvinbryanantia formatexigens在体外和具有突出糖解细菌的gnotobiotic小鼠肠道中的代谢潜力。为此，我们开发了一种普遍适用的方法，用于表达的微生物mRNA（微生物RNA-Seq）的多重测序，并结合代谢物的质谱分析，表明这些生物体具有不同的底物利用模式。B。hydrogenotrophica靶向脂肪族和芳香族氨基酸。它通过消耗还原当量来提高发酵效率，从而保持高NAD+/NADH比率并促进乙酸盐生产。相比之下，M。甲酸氧化酶消耗低聚糖，不影响肠道的氧化还原状态，并提高琥珀酸的产量。这些发现对于那些希望以调节能量收获的方式操纵肠道微生物群落的氢经济的人具有战略意义。
Fermenting microbial communities generate hydrogen; its removal through the production of acetate, methane, or hydrogen sulfide modulates the efficiency of energy extraction from available nutrients in many ecosystems. We noted that pathway components for acetogenesis are more abundantly and consistently represented in the gut microbiomes of monozygotic twins and their mothers than components for methanogenesis or sulfate reduction and subsequently analyzed the metabolic potential of two sequenced human gut acetogens, Blautia hydrogenotrophica and Marvinbryantia formatexigens in vitro and in the intestines of gnotobiotic mice harboring a prominent saccharolytic bacterium. To do so, we developed a generally applicable method for multiplex sequencing of expressed microbial mRNAs (microbial RNA-Seq) and, together with mass spectrometry of metabolites, showed that these organisms have distinct patterns of substrate utilization. B. hydrogenotrophica targets aliphatic and aromatic amino acids. It increases the efficiency of fermentation by consuming reducing equivalents, thereby maintaining a high NAD+/NADH ratio and boosting acetate production. In contrast, M. formatexigens consumes oligosaccharides, does not impact the redox state of the gut, and boosts the yield of succinate. These findings have strategic implications for those who wish to manipulate the hydrogen economy of gut microbial communities in ways that modulate energy harvest.