Dual Fucci: A New Transgenic Line for Studying the Cell Cycle from Embryos to Adults

Dual Fucci: A New Transgenic Line for Studying the Cell Cycle from Embryos to Adults
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DOI:
10.1089/zeb.2014.0986
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发表时间:
2014-04-01
期刊:
影响因子:
2
通讯作者:
Kimelman, David
Kimelman, David
中科院分区:
生物学4区
文献类型:
--
作者:
Bouldin, Cortney M.;Kimelman, David

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而原来的系统需要杂交两个不同的鱼,以产生胚胎表达两个报告转基因,新的系统,称为双Fucci,表达两个报告从一个单一的转基因(图1),从而允许杂交到其他转基因系(例如,报告线和热休克诱导线)。此外,本发明还提供了一种方法,
Whereas the original system required crossing two different fish to produce embryos expressing both reporter transgenes, the new system, called Dual Fucci, expresses both reporters from a single transgene (Fig. 1), thus permitting crosses to other transgenic lines (eg, reporter lines and heat-shock inducible lines). In addition,