Nitratireductor aquibiodomus gen. nov., sp. nov., a novel alpha-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada).
Nitratireductor aquibiodomus gen. nov., sp. nov., a novel alpha-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada).
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DOI：
10.1099/ijs.0.02793-0
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发表时间：
2004
影响因子：
2.8
通讯作者：
N. Labbé;S. Parent;R. Villemur
中科院分区：
文献类型：
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作者：
N. Labbé;S. Parent;R. Villemur
蒙特利尔生物圆顶运行一个甲醇进料的脱氮系统，处理其三百万升海洋生态系统中的水。在TSA和R2A琼脂上分离到一株未知细菌，命名为NL21T。该微生物为革兰氏阴性、杆状（1x3微米）兼性需氧菌。在R2A琼脂上的最佳生长条件是30 - 35 ℃，pH 7 - 7.5和1%（w/w）NaCl。16S rDNA序列的系统发育分析表明，菌株NL21 T形成了一个新的谱系在家庭的Phyllobacteriaceae的α 2亚群的变形菌。最接近的属是Aminobacterium、Pseudaminobacterium、Mesorhizobium和Defluvibacter。主要的细胞脂肪酸是C（18：1）omega7c（75%）、C（19：0）omega8c环丙烷（9.4%）和C（18：0）（4.2%）。菌株NL21T的DNA G + C含量（57 mol %）不同于"叶杆菌科"的所有其它已描述成员的DNA G + C含量（60 - 64 mol %）。菌株NL21T将硝酸盐还原为亚硝酸盐，但不将亚硝酸盐还原为氮气。只有少数糖和氨基酸可以作为碳源。菌株NL21T能够在没有盐的情况下生长，并且耐受高达5%的NaCl。系统发育分析以及生理生化试验表明，菌株NL 21 T不同于叶杆菌科所有已发表的有效名称的成员。因此，菌株NL21 T代表一个新属，其名称为Nitratireductor aquibiodomus gen.nov.，nov.，模式菌株为NL21T（= DSM 15645T = ATCC BAA-762T）。
The Montreal Biodome operates a methanol-fed denitrification system that treats the water in its three million litre marine mesocosm. An unknown bacterium, named strain NL21T, was isolated from this system on TSA and R2A agar. The organism is a Gram-negative, rod-shaped (1x3 microm) facultative aerobe. Optimal growth conditions on R2A agar are 30-35 degrees C, pH 7-7.5 and 1% (w/w) NaCl. Phylogenetic analysis of the 16S rDNA sequence reveals that strain NL21T forms a novel lineage in the family 'Phyllobacteriaceae' within the alpha2 subgroup of the Proteobacteria. The closest related genera are Aminobacter, Pseudaminobacter, Mesorhizobium and Defluvibacter. Major cellular fatty acids are C(18:1)omega7c (75%), C(19:0)omega8c cyclopropane (9.4%) and C(18:0) (4.2%). The DNA G+C content of strain NL21T (57 mol%) differs from those of all other described members of the 'Phyllobacteriaceae' (60-64 mol%). Strain NL21T reduces nitrate to nitrite, but does not reduce nitrite to nitrogen gas. Only a few sugars and amino acids can serve as carbon sources. Strain NL21T is able to grow without salt and tolerates up to 5% NaCl. Phylogenetic analysis, as well as physiological and biochemical tests, showed that strain NL21T was different from all other members of the 'Phyllobacteriaceae' with validly published names. Strain NL21T therefore represents a novel genus, for which the name Nitratireductor aquibiodomus gen. nov., sp. nov. is proposed, with the type strain NL21T (=DSM 15645T=ATCC BAA-762T).