Facile preparation of nanosilver particles with excellent antimicrobial activity via release of ionic silver
Facile preparation of nanosilver particles with excellent antimicrobial activity via release of ionic silver
复制标题
通过释放离子银轻松制备具有优异抗菌活性的纳米银颗粒
DOI：
10.1166/jnn.2017.12671
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Qingang Hu
中科院分区：
文献类型：
--
作者：
Xuna Tang;Dawei Guo;Shanxiang Jiang;Ning Gu;Yanhong Ni;Jiuyu Ge;Qingang Hu
纳米银粒子（SNPs）由于其优异的抑菌和杀菌作用，在包括医学在内的各个领域得到了广泛的应用。利用环保的溶剂体系和环保的还原剂制备snp具有重要的意义。在本研究中，没食子酸被用作还原剂和稳定剂，在温和的环境条件下合成snp。透射电镜（TEM）图像显示，SNPs呈近似球形，平均直径为13.81±2.21 nm。所得SNPs的吸光度峰明显，最大波长为400.5 nm，表明形成了小而高度单分散的SNPs。对这些snp对多种常见病原菌的抑菌潜力进行了评价。结果表明，微生物对snp的敏感性因微生物种类而异。其中革兰氏阴性菌对snp的敏感性高于革兰氏阳性菌。此外，银离子螯合剂n -乙酰- l-半胱氨酸（NAC）预处理对大肠杆菌和铜绿假单胞菌（P. aeruginosa）具有保护作用，而抗氧化应激剂l-抗坏血酸预处理对snp的抑菌作用无显著影响。这些数据表明，离子银的释放，而不是活性氧（ROS），在SNPs的抗菌作用中起关键作用。综上所述，本研究为简单合成具有良好抗菌潜力的snp提供了一种环境友好的技术。
Nanosilver particles (SNPs) have been widely exploited in various fields, including the medical sciences due to their excellent inhibitory and bactericidal effects. It is of great importance to prepare SNPs using green synthesis that has environmentally acceptable solvent systems and eco-friendly reducing agents. In the current study, gallic acid was employed as both a reducing agent and a stabilizing agent to synthesize SNPs at mild ambient conditions. The image of transmission electron microscopy (TEM) showed that SNPs exhibited approximately spherical shape with the average diameter of 13.81±2.21 nm. The absorbance peak of obtained SNPs was sharp with the maximum wavelength of 400.5 nm by ultraviolet-visible (UV-vis) spectroscopy, suggesting the formation of small and highly monodispersed SNPs. The antimicrobial potential of the SNPs was evaluated against multiple common pathogenic microbes. The results indicated that the microbial sensitivity to the SNPs was found to vary depending on the microbial species. Among them, the gram-negative bacteria exhibited more sensitive toward SNPs than the gram-positive bacteria. In addition, the N-acetyl-L-cysteine (NAC), a silver ion chelator, pretreatment could protect the E. coli and P. aeruginosa from the SNPs inhibition, while the pretreatment of the L-ascorbic acid, an antioxidant against oxidative stress, did not significantly influence the antibacterial effects of the SNPs. These data suggested that the ionic silver release, but not reactive oxygen species (ROS), played a key role in the antimicrobial effect of the SNPs. To sum up, this study provides an environmentally friendly technique for facile synthesis of SNPs with excellent antibacterial potential.