SATB1 promotes tumor metastasis and invasiveness in oral squamous cell carcinoma
SATB1 promotes tumor metastasis and invasiveness in oral squamous cell carcinoma
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SATB1促进口腔鳞状细胞癌的肿瘤转移和侵袭
DOI：
10.1111/odi.12602
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发表时间：
2017-03-01
期刊：
影响因子：
3.8
通讯作者：
Sun, Z-J
中科院分区：
文献类型：
--
作者：
Li, Y-C;Bu, L-L;Sun, Z-J
本研究旨在探讨SATB 1在口腔鳞状细胞癌（OSCC）中的表达及其在OSCC侵袭和转移中的作用。结果SATB 1在人口腔鳞癌细胞系SCC 25和Cal 27中高表达，其核质比与患者的预后密切相关。在体外研究中，在SATB 1敲低的细胞中观察到细胞运动性降低、侵袭性降低、上皮向间质转化（EMT）标记物（N-钙粘蛋白和β-连环蛋白）的表达以及上皮标记物的表达升高。SATB 1的耗竭也恢复了鹅卵石样的形态在TGF-1-treated cells.ConclusionsThese findings suggest SATB 1 may play an important role in OSCC invasiveness and metastasis.
ObjectiveOur aim is to evaluate the expression of SATB1 in human oral squamous cell carcinomas (OSCC) and its role in the invasiveness and metastasis of OSCC.Subjects and methodsA human OSCC tissue microarray was used to evaluate the expression pattern of SATB1. SATB1 mRNA knockdown was performed in human OSCC cell lines SCC25 and Cal27 to assess the function of SATB1 in the invasiveness and metastasis of OSCC.ResultsSATB1 is highly expressed in human OSCC determined by immunohistochemistry, and its nuclear/cytoplasmic ratio of histoscore is significantly correlated with patients' prognosis. Reduced cell motility, invasiveness, expression of epithelial to mesenchymal transition (EMT) markers (N-cadherin and -catenin), and elevated expression of epithelial markers were observed in SATB1-knockdown cells in invitro studies. Depletion of SATB1 also restored a cobblestone-like morphology in TGF-1-treated cells.ConclusionsThese findings suggest SATB1 may play an important role in OSCC invasiveness and metastasis.