Predominance of ST8 and CC1/spa-t1784 methicillin-resistant Staphylococcus aureus isolates in Japan and their genomic characteristics
Predominance of ST8 and CC1/spa-t1784 methicillin-resistant Staphylococcus aureus isolates in Japan and their genomic characteristics
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日本 ST8 和 CC1/spa-t1784 耐甲氧西林金黄色葡萄球菌的优势及其基因组特征
DOI：
10.1016/j.jgar.2022.01.011
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发表时间：
2022
影响因子：
4.6
通讯作者：
Kikuchi Ken
中科院分区：
文献类型：
--
作者：
Ogura Kohei;Kaji Daiki;Sasaki Masakazu;Otsuka Yoshihito;Takemoto Norihiko;Miyoshi-Akiyama Tohru;Kikuchi Ken
目的耐甲氧西林金黄色葡萄球菌（MRSA）已成为全球严重的流行病学问题。在这项研究中，我们的目的是调查最近分离的MRSA类型，并确定其characteristic.MethodsWe收集了164株分离自13家医院位于东京和周边地区。除了耐药性检测外，我们还对流行的MRSA克隆进行了全基因组测序，并分析了它们的基因组特征，如耐药性基因、毒力因子基因、结果SCCmec Ⅳ型MRSA的多位点测序分型结果显示51%属于克隆复合物1（CC 1），葡萄球菌蛋白A基因（spa）分型结果显示91%属于克隆复合物1（CC 1），而SCCmec Ⅳ型MRSA的基因型结果显示91%属于克隆复合物1（CC 1），SPA基因分型结果显示91%属于克隆复合物1（CC 1），SPA基因分型结果显示91%属于克隆复合物1（CC 1），SPA基因分型结果显示91%属于克隆复合物1（CC 1），SPA基因分型结果显示91%属于克隆复合物1（SPA 1），SPA基因分型结果显示91%属于克隆复合物1（CC 1），SPA基因分型结果显示91%属于克隆复合物1（SPA 1），SPA基因分型结果显示91%属于克隆复合物1（CC 1），SPA基因分型结果显示91%属于克隆复合物1（SPA 1），SPA基因分型结果显示91%属于克隆复合物1（CC 1）CC1菌株中有10株属于t1784型。这些CC1/t1784菌株均具有红霉素耐药蛋白、壮观霉素9-腺苷酰转移酶和葡萄球菌肠毒素（SEA、SEI、SEM）基因，但不具有Panton-Valentine杀白细胞素pvl基因。9个CC1/t1784分离株的完整基因组分析表明，它们共享一个完整的噬菌体，其中携带没有注释的毒力因子基因，除了两个编码一个假设的膜蛋白和磷壁酸生物合成蛋白。CC1/t1784分离株之间没有观察到显著的基因组重排。ConclusionThese data and previous reports indicate that this CC1/t1784 clone has been expanding rapidly in Japan without genomic changes.
ObjectivesMethicillin-resistantStaphylococcus aureus(MRSA) has become a serious epidemiologic problem worldwide. In this study, we aimed to investigate recently isolated MRSA types and determine their characteristics.MethodsWe collected 164 strains isolated from 13 hospitals located in Tokyo and surrounding prefectures. In addition to drug resistance tests, we sequenced whole genomes of the prevalent MRSA clones and analysed their genomic characteristics, such as drug resistance genes, virulence factor genes, and genome arrangements.ResultsMultilocus sequencing typing showed that 51% of the SCCmecⅣ MRSA isolates belonged to clonal complex 1 (CC1).Staphylococcusprotein A gene (spa) typing showed that 91% of these CC1 isolates could be categorised as t1784 type. These CC1/t1784 isolates possessed genes encoding erythromycin resistance protein, spectinomycin 9-adenylyltransferase, and staphylococcal enterotoxins (SEA, SEI, SEM), but not thepvlgene encoding Panton-Valentine leukocidin. Complete genomic analysis of nine CC1/t1784 isolates showed that they shared an intact phage, which carried no annotated virulence factor genes except for two encoding a hypothetical membrane protein and a teichoic acid biosynthesis protein. No significant genomic rearrangements were observed among the CC1/t1784 isolates.ConclusionThese data and previous reports indicate that this CC1/t1784 clone has been expanding rapidly in Japan without genomic changes.