The functional microarchitecture of the mouse barrel cortex
The functional microarchitecture of the mouse barrel cortex
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DOI：
10.1371/journal.pbio.0050189
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发表时间：
2007-07-01
期刊：
影响因子：
9.8
通讯作者：
Svoboda, Karel
中科院分区：
文献类型：
--
作者：
Sato, Takashi R.;Gray, Noah W.;Svoboda, Karel
大脑皮层地图由有序排列的功能柱组成，是大脑皮层组织的标志。然而，在单个神经元水平上的皮层映射的微组织尚不清楚，这主要是因为现有映射技术的局限性。在这里，我们使用钙离子指示剂的批量加载结合双光子显微镜来成像活体小鼠桶状皮质2/3层(L)的多个单一神经元的活动。我们开发了在L2/3中大约一半的被成像神经元中可靠地检测单个动作电位的方法。这使得我们能够测量胡须偏转后的尖峰概率，从而映射具有已知空间关系的多个神经元的胡须选择性。在神经元群体的水平上，胡须地图在大脑皮层表面、桶内和桶之间平稳变化。然而，同时记录的单个神经元的胡须选择性差异很大，即使是最近的邻居也是如此。在跨越多个皮质柱的距离上，成对神经元之间的试验相关性很高。我们的数据表明，单个神经元的反应特性是由高度特异的亚柱状回路和新皮质的瞬时内在状态决定的。
Cortical maps, consisting of orderly arrangements of functional columns, are a hallmark of the organization of the cerebral cortex. However, the microorganization of cortical maps at the level of single neurons is not known, mainly because of the limitations of available mapping techniques. Here, we used bulk loading of Ca2+ indicators combined with two-photon microscopy to image the activity of multiple single neurons in layer (L) 2/3 of the mouse barrel cortex in vivo. We developed methods that reliably detect single action potentials in approximately half of the imaged neurons in L2/3. This allowed us to measure the spiking probability following whisker deflection and thus map the whisker selectivity for multiple neurons with known spatial relationships. At the level of neuronal populations, the whisker map varied smoothly across the surface of the cortex, within and between the barrels. However, the whisker selectivity of individual neurons recorded simultaneously differed greatly, even for nearest neighbors. Trial-to-trial correlations between pairs of neurons were high over distances spanning multiple cortical columns. Our data suggest that the response properties of individual neurons are shaped by highly specific subcolumnar circuits and the momentary intrinsic state of the neocortex.