Expression of Nephrin, Podocin, α-Actinin-4 and α3-Integrin in Canine Renal Glomeruli
Expression of Nephrin, Podocin, α-Actinin-4 and α3-Integrin in Canine Renal Glomeruli
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DOI：
10.1016/j.jcpa.2011.01.007
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发表时间：
2011-08-01
影响因子：
0.8
通讯作者：
Shirota, K.
中科院分区：
文献类型：
--
作者：
Kobayashi, R.;Kamiie, J.;Shirota, K.
尚未在犬中研究足细胞的生物学特征对蛋白尿发病机制的影响。本研究旨在探讨nephrin、podocin、α-actinin-4和α 3-integrin在犬肾小球中的表达和定位。从五只正常成年比格犬的肾脏收集肾皮质组织。免疫印迹和免疫荧光显微镜显示，在犬肾小球特异性表达和定位的四种蛋白质。逆转录聚合酶链反应检测肾小球中编码这四种分子的基因的表达。这项研究的结果将允许未来的探索足细胞损伤及其参与蛋白质泄漏从毛细血管壁犬肾小球疾病。(C)2011爱思唯尔有限公司保留所有权利。
The biological features of podocytes that contribute to the pathogenesis of proteinuria have not been investigated in dogs. The aim of this study was to investigate the expression and localization of nephrin, podocin, alpha-actinin-4 and alpha 3-integrin in canine renal glomeruli. Renal cortical tissue was collected from the kidneys of five normal adult beagles. Western blotting and immunofluorescence microscopy revealed specific expression and localization of the four proteins in canine glomeruli. Expression of genes encoding the four molecules in isolated glomeruli was detected by reverse transcriptase polymerase chain reaction. The results of this study will permit future exploration of podocyte injury and its involvement in protein leakage from the capillary wall in canine glomerular diseases. (C) 2011 Elsevier Ltd. All rights reserved.