Assays of Protonemal Growth Responses in Physcomitrella patens Under Blue- and Red-Light Stimuli

Assays of Protonemal Growth Responses in Physcomitrella patens Under Blue- and Red-Light Stimuli
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蓝光和红光刺激下小立碗藓的质子生长反应测定

DOI:
10.1007/978-1-4939-9015-3_4
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发表时间:
2019
期刊:
Methods Mol Biol
影响因子:
--
通讯作者:
Kawaide Hiroshi
Kawaide Hiroshi
中科院分区:
--
文献类型:
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作者:
Miyazaki Sho;Nakajima Masatoshi;Kawaide Hiroshi

文献摘要

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无种子陆地植物小壶藓是苔藓类植物发育、细胞和分子生物学研究的模式物种,也是进行遗传分析的模式物种。此前,研究表明野生型p。专利显示出一种独特的光形态发生行为,其中叶绿体细丝在与蓝光光源相反的方向生长。在这里,我们描述了生物测定系统,可用于研究避光反应以及p光形态发生调控的其他方面。在红光和蓝光光源下生长的植物。
The non-seed land plantPhyscomitrella patensis a model species for developmental, cellular, and molecular biology studies in mosses and also for performing genetic analyses. Previously, it was shown that wild-typeP. patensdisplays a unique photomorphogenetic behavior, in which chloronemal filaments grow in the opposite direction to a blue-light source. Here, we describe bioassay systems that can be used to study light avoidance responses as well as other aspects of photomorphogenetic regulation inP. patensgrown under red- and blue-light sources.