CYP4Z1 3′UTR represses migration of human breast cancer cells
CYP4Z1 3′UTR represses migration of human breast cancer cells
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CYP4Z1 3'UTR抑制人乳腺癌细胞的迁移
DOI：
10.1016/j.bbrc.2016.08.048
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发表时间：
2016-09-16
影响因子：
3.1
通讯作者：
Xi, Tao
中科院分区：
文献类型：
--
作者：
Wang, Bingyu;Zheng, Lufeng;Xi, Tao
为了研究CYP4Z1 3' UTR对乳腺癌细胞迁移的影响，采用一系列测定来证实CYP4Z1 3' UTR的过表达可以抑制MCF-7和MDA-MB-231细胞的迁移和粘附能力。 EMT（上皮间质转化）相关蛋白受 CYP4Z1 3' UTR 调节。波形蛋白、MMP-2 和 MMP-9 等间质标记物下调，而 E-钙粘蛋白的表达则因 CYP4Z1 3' UTR 过表达而上调。值得注意的是，应用荧光素酶报告基因和 qRT-PCR 测定来验证 CYP4Z1 3' UTR 是 miR-9 的潜在靶标。此外，我们的结果表明，CYP4Z1 3' UTR 以 miRNA 依赖性方式抑制 E-钙粘蛋白的表达。结合我们之前的研究，我们发现了CYP4Z1和E-cadherin之间的潜在联系。因此，这些初步数据表明CYP4Z1 3'UTR可以通过充当E-钙粘蛋白的ceRNA来抑制乳腺癌细胞的迁移和EMT。 (C) 2016 Elsevier Inc. 保留所有权利。
To investigate the effects of CYP4Z1 3 ' UTR in migration of breast cancer cells, a series of assays were used to confirm that overexpression of CYP4Z1 3 ' UTR could suppress the capacity of migration and adhesion of MCF-7 and MDA-MB-231 cells. EMT (Epithelial-mesenchymal transition)-related proteins were regulated by CYP4Z1 3 ' UTR. Mesenchyma markers like Vimentin, MMP-2, and MMP-9 were down regulated, while the expression of E-cadherin was up -regulated with CYP4Z1 3 ' UTR overexpression. Notably, luciferase reporter and qRT-PCR assays were applied to verify that CYP4Z1 3 ' UTR was the potential target of miR-9. In addition, our results showed that CYP4Z1 3 ' UTR repressed the expression of E-cadherin in a miRNA-dependent manner. Combining with our previous study, we have discovered the underlying link between CYP4Z1 and E-cadherin. Therefore, those preliminary data suggest that CYP4Z1 3 ' UTR could inhibit the migration and EMT of breast cancer cells via acting as a ceRNA for E-cadherin. (C) 2016 Elsevier Inc. All rights reserved.