Molecular epidemiology of attachment glycoprotein(G) gene in respiratory syncytial virus in children with acute respiratory infection in Japan in 2009/2010
Molecular epidemiology of attachment glycoprotein(G) gene in respiratory syncytial virus in children with acute respiratory infection in Japan in 2009/2010
复制标题
2009/2010年日本急性呼吸道感染患儿呼吸道合胞病毒附着糖蛋白(G)基因分子流行病学分析
DOI：
10.1099/jmm.0.041137-0
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发表时间：
2012
影响因子：
3
通讯作者：
Masato Tashi
中科院分区：
文献类型：
--
作者：
Ayako Yoshida1;Naoko Kiyota;Miho Kobayashi;Koichi Nishimura;Rika Tsutsui;Hiroyuki Tsukagoshi;Eiko Hirano;Norio Yamamoto;Akihide Ryo;Mika Saitoh;Seiya Harada;Osamu Inoue;Kunihisa Kozawa;Ryota Tanaka;Masahiro Noda;Nobuhiko Okabe;Masato Tashi
本研究对2009/2010年季节在50例日本急性呼吸道感染（ARI）儿童中检测到的呼吸道合胞病毒（RSV）糖蛋白（G）基因进行了详细的遗传分析。邻区连接法构建的系统发育树显示，A亚群34株，B亚群B 16株。属于A亚组和B亚组的菌株分别进一步细分为GA 2和BA。这些菌株之间的核苷酸和推导的氨基酸序列同源性较高（> 90%）。 推测的氨基酸序列表明，这些菌株的G蛋白的C-末端第3高变区发生了相对较高的频率的氨基酸替换。此外，还估计了一些正选择位点。结果表明，2009/2010年日本儿童中基因型为GA 2和BA的RSV与ARI相关。
This study performed a detailed genetic analysis of the glycoprotein (G) gene of respiratory syncytial virus (RSV) detected in 50 Japanese children with acute respiratory infection (ARI) in the 2009/2010 season. A phylogenetic tree constructed by the neighbour-joining method showed that 34 and 16 of the RSV strains could be classified into subgroups A and B, respectively. Strains belonging to subgroups A and B were further subdivided into GA2 and BA, respectively. The nucleotide and deduced amino acid sequence identities were relatively high among these strains (>90 %). The deduced amino acid sequences implied that a relatively high frequency of amino acid substitutions occurred in the C-terminal 3rd hypervariable region of the G protein in these strains. In addition, some positively selected sites were estimated. The results suggest that RSV with genotypes GA2 and BA was associated with ARI in Japanese children in 2009/2010.