Memory T Cells (CD45RO) Role and Evaluation in Pathogenesis of Lichen Planus and Lichenoid Mucositis
Memory T Cells (CD45RO) Role and Evaluation in Pathogenesis of Lichen Planus and Lichenoid Mucositis
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DOI：
10.7860/jcdr/2017/26866.9930
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发表时间：
2017-05-01
影响因子：
0.2
通讯作者：
Janane, Murali
中科院分区：
文献类型：
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作者：
Devi, Mani;Vijayalakshmi, Dhanaraj;Janane, Murali
简介：记忆T细胞具有在静止状态下存活更长时间的能力，并且负责随后暴露于抗原时的快速反应。目的：应用CD 45 RO单克隆抗体免疫组化法鉴定口腔扁平苔藓（OLP）、苔藓样粘膜炎（LM）和正常粘膜（NM）中的记忆性T细胞，并对其进行评价。30例经临床和病理确诊的口腔扁平苔藓（OLP）和15例淋巴结转移（LM），10例淋巴结转移（NM）采用生物素-链霉亲和素免疫组化方法进行CD 45 RO单克隆抗体染色。结果：CD 45 RO在OLP结缔组织层的表达（53.3%强染色）高于LM（20%强染色），而在NM无强染色。研究组之间的染色强度模式的差异具有统计学意义（p=0.014）。结论：本研究表明，在LP中的记忆T细胞比LM中的记忆T细胞有统计学意义的上升，表明可能不同的免疫病理机制。
Introduction: Memory T cells have the ability to survive in a quiescent state for longer periods and are responsible for the rapid responses on subsequent exposure to antigen. Analyzing memory T cells in Oral Lichen planus (OLP) and Lichenoid Mucositis (LM) suggest that these cells may play a role in the immunopathogenic mechanisms.Aim: To identify and evaluate Memory T cells in Lichen Planus (LP), Lichenoid Mucositis ( LM) and Normal Mucosa (NM) using CD45RO monoclonal antibody immunohistochemically.Materials and Methods: A total of 30 cases (15 cases of OLP and 15 cases of LM) clinically and histopathologically diagnosed, and 10 cases of NM were stained for CD45RO monoclonal antibody, immunohistochemically using Biotin Streptavidin method. Staining intensity of CD45RO expression was statistically analysed using Chi-square Test.Results: The present study demonstrated a higher expression of CD45RO in connective tissue layer of OLP (53.3% intense staining) when compared to LM (20% intense staining) and no intense staining in NM. The difference in staining intensity pattern between the study groups was statistically significant (p=0.014).Conclusion: This study demonstrates a statistically significant rise in memory T cells in LP than in LM, indicating the possible different immunopathogenic mechanisms.