Curcumin in plasma and urine: quantitation by high-performance liquid chromatography
Curcumin in plasma and urine: quantitation by high-performance liquid chromatography
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DOI：
10.1016/s1570-0232(02)00714-6
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发表时间：
2003-01-05
影响因子：
3
通讯作者：
Rock, CL
中科院分区：
文献类型：
--
作者：
Heath, DD;Pruitt, MA;Rock, CL
姜黄素是姜黄植物的衍生物，在食品工业中被广泛使用。它是咖喱粉的主要成分，研究表明姜黄素可以预防癌症和其他慢性疾病。我们开发了一种可靠的测定血浆和尿液中姜黄素的自动化分析方法。用乙酸乙酯/甲醇有机溶剂从0.2 ml样品中提取姜黄素，内标β -17-雌二醇醋酸酯。分析采用反相C-18柱和262 nm的紫外检测。已对性能特征进行了评估。在0.2 ~ 7.0马克杯/毫升范围内呈线性。日内和日内测定的变异系数为
Curcumin, a derivative of the plant Curcuma longa, is used extensively in the food industry. It is a major component of curry powder, and research has shown that curcumin may prevent cancer and other chronic diseases. We have developed a robust automated analytical method for the determination of curcumin in plasma and urine. The method involves extracting the curcumin from 0.2 ml sample volume with ethyl acetate/methanol organic solvents, and use of an internal standard, beta-17-estradiol acetate. Analysis utilizes a reversed-phase C-18 column and UV detection at 262 nm. Performance characteristics have been assessed. The assay is linear from 0.2 to 7.0 mug/ml. The coefficient of variation for intra- and inter-day assays is