Preparation and antioxidant activity of Radix Astragali residues extracts rich in calycosin and formononetin
Preparation and antioxidant activity of Radix Astragali residues extracts rich in calycosin and formononetin
复制标题
DOI：
10.1016/j.bej.2011.04.015
复制
发表时间：
2011-09-15
影响因子：
3.9
通讯作者：
Efferth, Thomas
中科院分区：
文献类型：
--
作者：
Chen, Cai-Yun;Zu, Yuan-Gang;Efferth, Thomas
黄芪是我国传统中药的优良来源，也是著名的滋补食品。为充分利用板蓝根，采用多种大孔树脂对板蓝根药渣中毛蕊异黄酮和芒柄花素进行富集，比较了HPD 500树脂对毛蕊异黄酮和芒柄花素的富集分离效果。选择35 ℃是因为其在静态吸附过程中的最高采用容量，这可以从吸附等温线获得。并进行了动态吸附和脱附实验，优化了操作参数。经HPD-500树脂处理后，毛蕊异黄酮和芒柄花素的含量分别提高了4.41倍和4.60倍，回收率分别为75.16%和77.06%。抗氧化活性的研究表明，60%乙醇提取物具有更好的自由基清除活性（IC 50 0.03 mg/mL）比粗提物（IC 50 0.05 mg/mL）。本研究建立了一种快速、有效的方法，为规模化制备毛蕊异黄酮和芒柄花素奠定了基础。(C)2011 Elsevier B. V.保留所有权利。
Radix Astragali is considered an excellent source of Traditional Chinese Medicine and a kind of famous restorative food. To make full usage of Radix Astragali, calycosin and formononetin were enriched through various macroporous resins from its residues, HPD500 resin, compared with other resins, offered the best effectiveness for the enrichment and separation of compounds. 35 degrees C was selected for its highest adoption capacity during static adsorption process, which can be obtained from adsorption isotherms. And then dynamic adsorption and desorption tests were carried out to optimize the operating parameters. After one run treatment with HPD500 resin, the contents of calycosin and formononetin in the product were 4.41-fold and 4.60-fold with recovery yields of 75.16% and 77.06%, respectively. Investigations on the antioxidant activity showed that eluate with 60% ethanol exhibited better free radical-scavenging activity (IC50 0.03 mg/mL) than crude extracts (IC50 0.05 mg/mL). Thus, this fast and effective method was established and could be a promising for the large-scale preparation of calycosin and formononetin from Radix Astragali residues. (C) 2011 Elsevier B.V. All rights reserved.