Dendritic Cell-Based Vaccine Against Fungal Infection
Dendritic Cell-Based Vaccine Against Fungal Infection
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基于树突状细胞的真菌感染疫苗
DOI：
10.1007/978-1-4939-3387-7_30
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Kinjo Y.
中科院分区：
文献类型：
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作者：
Ueno K;Urai M;Ohkouchi K;Miyazaki Y;Kinjo Y.
几种致病真菌，包括隐球菌、荚膜组织胞浆菌、粗球孢子菌和马尔尼菲青霉菌，在免疫功能正常的人类中引起严重的感染性疾病。然而，目前，预防性和治疗性疫苗尚未在临床上使用。特别是C. GATTII是一种新出现的病原体，迄今为止针对该病原体的保护性免疫尚未得到很好的表征。实验疫苗如组分活疫苗和减毒活疫苗已被用作研究针对真菌感染的保护性免疫的工具。最近，我们开发了一种基于树突状细胞（DC）的疫苗，以研究针对高度嗜酸性粒细胞（HKC）肺部感染的保护性免疫。在温哥华岛爆发期间，临床上从感染患者的支气管冲洗液中分离的gattiisstrain R265。在这种方法中，骨髓来源的DC（BMDC）用热灭活的C脉冲。gattil，然后在肠道内感染之前转移到小鼠中。这种DC疫苗显著增加了肺和脾中产生白细胞介素17 A（IL-17 A）、干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）的T细胞，并改善了C.胃肠道感染。这种方法可能会导致控制致命真菌感染的新方法的发展。在本章中，我们描述了DC疫苗的制备过程和小鼠肺部感染模型，用于分析针对C的免疫应答。加提。
Several pathogenic fungi, includingCryptococcus gattii,Histoplasma capsulatum,Coccidioides immitis, andPenicillium marneffei, cause serious infectious diseases in immunocompetent humans. However, currently, prophylactic and therapeutic vaccines are not clinically used. In particular,C. gattiiis an emerging pathogen and thus far protective immunity against this pathogen has not been well characterized. Experimental vaccines such as component and attenuated live vaccines have been used as tools to study protective immunity against fungal infection. Recently, we developed a dendritic cell (DC)-based vaccine to study protective immunity against pulmonary infection by highly virulentC. gattiistrain R265 that was clinically isolated from bronchial washings of infected patients during the Vancouver Island outbreak. In this approach, bone marrow-derived DCs (BMDCs) are pulsed with heat-killedC. gattiiand then transferred into mice prior to intratracheal infection. This DC vaccine significantly increases interleukin 17A (IL-17A)-, interferon gamma (IFN-γ)-, and tumor necrosis factor alpha (TNF-α)-producing T cells in the lungs and spleen and ameliorates the pathology, fungal burden, and mortality followingC. gattiiinfection. This approach may result in the development of a new means of controlling lethal fungal infections. In this chapter, we describe the procedures of DC vaccine preparation and murine pulmonary infection model for analysis of immune response againstC. gattii.