CHROMAFFIN CELL CORTICAL ACTIN NETWORK DYNAMICS CONTROL THE SIZE OF THE RELEASE-READY VESICLE POOL AND THE INITIAL RATE OF EXOCYTOSIS
CHROMAFFIN CELL CORTICAL ACTIN NETWORK DYNAMICS CONTROL THE SIZE OF THE RELEASE-READY VESICLE POOL AND THE INITIAL RATE OF EXOCYTOSIS
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DOI：
10.1016/0896-6273(95)90291-0
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发表时间：
1995-02-01
期刊：
影响因子：
16.2
通讯作者：
TRIFARO, JM
中科院分区：
文献类型：
--
作者：
VITALE, ML;SEWARD, EP;TRIFARO, JM
形态学，生物化学和膜电容测量被用来研究皮质丝状肌动蛋白（F-肌动蛋白）的作用，在exoctyosis。静息嗜铬细胞的荧光和电子显微镜显示皮质肌动蛋白网络，排除分泌囊泡从质膜下区域。佛波酯（PMA）治疗破坏皮质F-肌动蛋白和增加的囊泡的数量在0-50 nm的subplasmalemmal区和刺激的儿茶酚胺释放的初始速率。在PMA预处理的细胞，膜电容的研究表明，在火车的前两个去极化与质膜融合的囊泡的数量增加。PMA不影响电压依赖性Ca ~（2+）内流。与质膜融合的囊泡的总数与占据0-50 nm皮质区的囊泡的数量相关性良好。因此，皮质F-肌动蛋白解体允许小泡易位到质膜，为胞吐做准备。
Morphological, biochemical, and membrane capacitance measurements were used to study the role of cortical filamentous actin (F-actin) in exoctyosis. Fluorescence and electron microscopy of resting chromaffin cells revealed a cortical actin network that excluded secretory vesicles from the subplasmalemmal area. Phorbol ester (PMA) treatment disrupted cortical F-actin and increased both the number of vesicles within the 0-50 nm subplasmalemmal zone and the initial rate of stimulated catecholamine release. In PMA-pretreated cells, membrane capacitance studies showed an increased number of vesicles fusing with the plasmalemma during the first two depolarizations of a train. PMA did not affect voltage-dependent Ca2+ influx. The total number of vesicles fused with the plasma membrane correlated well with the number of vesicles occupying the 0-50 nm cortical zone. Therefore, cortical F-actin disassembly allows translocation of vesicles to the plasmalemma in preparation for exocytosis.