Increased interleukin-12 release from peripheral blood mononuclear cells in nephrotic phase of minimal change nephrotic syndrome.
Increased interleukin-12 release from peripheral blood mononuclear cells in nephrotic phase of minimal change nephrotic syndrome.
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DOI：
10.7097/apt.200404.0077
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发表时间：
2004-04
期刊：
影响因子：
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通讯作者：
Ching‐Yuang Lin;J. Chien
中科院分区：
文献类型：
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作者：
Ching‐Yuang Lin;J. Chien
微小病变型肾病综合征(MCNS)的发病机制尚不清楚。多项研究表明，MCNS是一种细胞免疫的全身性疾病。IL-12是一种主要由巨噬细胞产生的多效性细胞因子，在Th1介导的免疫诱导中起主要作用。本研究的目的是探讨MCNS患者体内血、尿IL-12水平与体外IL-12产生的关系。检测20例肾病和缓解期MCNS患者外周血单个核细胞(PBMNCs)经内毒素刺激后体外产生IL-12，用明尼阿波利斯R&D系统公司生产的ELISA试剂盒检测血清和尿液IL-12水平。结果表明，患者血清IL-12水平及内毒素刺激的PBMNCs产生IL-12水平均显著高于正常对照组和缓解期患者。而肾病缓解期MCNS患者尿IL-12水平与正常对照组比较差异有统计学意义(P<0.05)。本研究证实了MCNS患者PBMNCs体外释放IL-12与体内血清IL-12水平的相关性及其与疾病活动性的关系。本研究有助于了解MCNS的发病机制。
The pathogenesis of minimal change nephrotic syndrome (MCNS) is still not clear. Several studies indicate that MCNS is a systemic disorder of cell-mediated immunity. IL-12 is a pleiotropic cytokine that is produced primarily by macrophage and plays a primary role in the induction of Th1-mediated immunity. The purpose of this study is to explore the relationship of in vivo IL-12 levels in both sera and urine, and in vitro IL-12 production in stable or nephrotic clinical condition patients with MCNS. In vitro IL-12 production was detected from LPS-stimulated peripheral blood mononuclear cells (PBMNCs) of 20 MCNS patients during nephrotic and remission stages, levels of sera and urinary IL-12 were measured by commercially available ELISA kits (R & D Systems, Minneapolis). The results showed sera IL-12 levels and LPS-stimulated IL-12 productions for PBMNCs were significantly increased as compared with those of normal controls and in remission stage. However, there was also statistical difference of urinary IL-12 levels among MCNS patients during nephrotic and remission stage and controls. This study demonstrates a correlation between in vitro IL-12 release by PBMNCs and in vivo sera IL-12 level from MCNS patients as well as correlation with disease activity. The study contributes to the understanding of the pathogenesis of MCNS.