Time-related increase in mitochondrial superoxide production, biomolecule damage and antioxidant enzyme activities in cortical astrocyte cultures
Time-related increase in mitochondrial superoxide production, biomolecule damage and antioxidant enzyme activities in cortical astrocyte cultures
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DOI：
10.1097/00001756-200208270-00005
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发表时间：
2002-08-27
期刊：
影响因子：
1.7
通讯作者：
Souza, DO
中科院分区：
文献类型：
--
作者：
Klamt, F;Gottfried, C;Souza, DO
根据衰老的自由基理论，生物衰老过程是由于氧化应激引起的机体内环境稳定的普遍失败而发展起来的。大脑特别容易受到氧化损伤，而星形胶质细胞主要负责其抗氧化防御。在这里，我们评估和比较酶的抗氧化活性，线粒体超氧化物的产生，和生物分子的氧化损伤新生Wistar大鼠皮层星形胶质细胞培养10-13或40-47天。我们发现，除了在成熟的星形胶质细胞培养物中的抗氧化酶活性的增加，脂质过氧化和蛋白质氧化的增加，可能是由于线粒体电子传递链超氧化物的产生增加。这可能表明防御机制的增加不足以避免成熟过程中的氧化生物分子损伤。
According to the free radical theory of aging, biological senescence processes develop from a general failure to maintain organism's homeostasis, probably due to oxidative stress. The brain is particularly susceptible to oxidative damage, and astrocytes are chiefly responsible for its antioxidant defense. Here we evaluated and compared the enzymatic antioxidant activities, mitochondrial superoxide production, and oxidative damage in biomolecule in cortex astrocytes from newborn Wistar rats maintained for 10-13 or 40-47 days in culture. We show that, besides an increase in antioxidant enzyme activities in matured astrocyte cultures, there was an increase in lipoperoxidation and in protein oxidation, probably due to an increase in mitochondrial electron transport chain superoxide production. This could indicate that the increasing in defense mechanisms was not sufficient to avoid oxidative biomolecule damage during maturation.