Expression and distribution of vanilloid receptor 1 (TRPV1) in the adult rat brain
Expression and distribution of vanilloid receptor 1 (TRPV1) in the adult rat brain
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DOI：
10.1016/j.molbrainres.2004.12.003
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发表时间：
2005-04-27
期刊：
影响因子：
--
通讯作者：
Blumberg, PM
中科院分区：
文献类型：
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作者：
Tóth, A;Boczán, J;Blumberg, PM
香草素受体（TRPV1或VR1）是各种疼痛刺激的分子整合者，包括辣椒素、酸和高温。它也可以被内源性配体激活，如大麻素1受体（CBI）激动剂anandamide。TRPV1在参与疼痛通路的感觉神经末梢被很好地表征。也有证据表明TRPV1在大脑中表达，但对其功能知之甚少。本研究利用市买特异性抗体研究TRPV1在大鼠大脑中的定位，发现TRPV1在海马、皮质、小脑、嗅球、中脑和后脑均有表达。免疫组化分析显示海马和皮层神经元细胞体和树突高表达。为了解决亚细胞定位的问题，使用了免疫电子显微镜。trpv1样染色在突触（大部分但不限于突触后树突棘）、星形胶质细胞端足和周细胞中检测到。综上所述，TRPV1的表达在大鼠大脑中分布广泛，在星形胶质细胞和周细胞以及神经元中都有表达。其定位与中枢神经系统内的多种功能一致，包括对脑血管的调节。Elsevier B.V.出版
The vanilloid receptor (TRPV1 or VR1) is a molecular integrator of various painful stimuli, including capsaicin, acid, and high temperature. It can also be activated by endogenous ligands, like the cannabinoid 1 receptor (CBI) agonist anandamide. TRPV1 is well characterized at the terminals of sensory nerves involved in the pain pathway. There is also evidence that TRPV1 is expressed in the brain but little is known about its function. Here, using commercially available specific antibodies to investigate the localization of TRPV1 in the brain of the rat, we report that TRPV1 was expressed in hippocampus, cortex, cerebellum, olfactory bulb, mesencephalon and hindbrain. Immunohistochemical analyses showed high expression in the cell bodies and dendrites of neurons in the hippocampus and in the cortex. To address the question of subcellular localization, immunoelectronmicroscopy was used. TRPV1-like staining was detected in the synapses (mostly, but not exclusively in post-synaptic dendritic spines), on the end feet of astrocytes and in pericytes. In summary, TRPV1 expression shows wide distribution in the brain of the rat, being found in astrocytes and pericytes as well as in neurons. Its localization is consistent with multiple functions within the central nervous system, including the regulation of brain vasculature. Published by Elsevier B.V.