Histological study of acute vocal fold injury in a rat model
Histological study of acute vocal fold injury in a rat model
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DOI：
10.1177/000348940611500406
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发表时间：
2006-04-01
影响因子：
1.4
通讯作者：
Bless, DM
中科院分区：
文献类型：
--
作者：
Tateya, T;Tateya, L;Bless, DM
目的：利用大鼠急性声带损伤模型，通过研究声带固有层细胞外基质成分的表达模式来表征声带创伤愈合。方法：27只Sprague-Dawley大鼠单侧进行声带剥离。分别于1、3、5、7、14天5个时间点摘取声带，用阿利新蓝染色进行组织学分析。三色的污渍。用免疫荧光法检测1型胶原蛋白和III型胶原蛋白的抗体。纤连蛋白。结果：第3天发生再上皮化，第14天完成。第3天形成肉芽组织。损伤声带第3天出现透明质酸和ⅰ型胶原蛋白，第5天达到峰值。然后下降。ⅲ型胶原蛋白和纤维连接蛋白在第1天出现，并在第3天之后的所有时间点持续增强。结论：这些细胞外基质成分的表达在第3 ~ 5天达到高峰。声带伤口愈合的特征与早期皮肤伤口愈合的特征相似。但在随后的重塑阶段有所不同。
Objectives: We used an acute vocal fold injury in a rat model to characterize vocal fold wound healing by studying the expression pattern of the extracellular matrix components in the vocal fold lamina propria.Methods: Vocal fold stripping was performed unilaterally in 27 Sprague-Dawley rats. The vocal folds were harvested at 5 time points (1, 3, 5, 7, and 14 days) and histologically analyzed by Alcian blue stain. trichrome stain. and immunofluorescence with antibodies to collagen type 1, collagen type III. and fibronectin.Results: Re-epithelialization occurred by day 3 and was complete by day 14. Granulation tissue was formed by day 3. Hyaluronic acid and collagen type I appeared in injured vocal folds by day 3, peaked at day 5. and thereafter decreased. Collagen type III and fibronectin appeared by day I and continued to be intense at all time points after day 3.Conclusions: These results suggest that the expression of these extracellular matrix components peaks in the period around days 3 to 5. and that the characteristics of wound healing in the vocal fold are similar to those in the skin in the early phases. but differ during the subsequent remodeling phase.