Peroxynitrite scavenging activity of herb extracts
Peroxynitrite scavenging activity of herb extracts
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DOI：
10.1002/ptr.904
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发表时间：
2002-06-01
影响因子：
7.2
通讯作者：
Chung, HY
中科院分区：
文献类型：
--
作者：
Choi, HR;Choi, JS;Chung, HY
过氧亚硝酸盐(ONOO-)是一种细胞毒物，对多种细胞成分，包括巯基、脂类、氨基酸和核苷酸具有很强的氧化作用，可导致细胞死亡、脂质过氧化、致癌和衰老。本研究的目的是对中草药中清除ONOO的成分进行表征。采用荧光分光光度法对28种中草药进行了ONOO清除活性的筛选。添加正宗ONOO-后，清除活性的效力顺序如下：金缕梅树皮；迷迭香；茉莉花茶；鼠尾草；滑榆树；黑核桃叶；安妮女王蕾丝；菩提花。提取物对ONOO的清除作用呈剂量依赖性。我们发现金缕梅(Hamamelis virginiana L.)在28种中草药中，树皮对ONOO-的清除作用最强。金缕梅单宁是金缕梅树皮的主要活性成分，具有很强的清除ONOO-的能力。提示金缕梅单宁可作为一种有效的过氧亚硝酸盐清除剂用于预防ONOO相关疾病。版权所有(C)2002 John Wiley Sons，Ltd.
Peroxynitrite (ONOO-) is a cytotoxicant with strong oxidizing properties toward various cellular constituents, including sulphydryls, lipids, amino acids and nucleotides and can cause cell death, lipid peroxidation, carcinogenesis and aging. The aim of this study was to characterize ONOO- scavenging constituents from herbs. Twenty-eight herbs were screened for their ONOO- scavenging activities with the use of a fluorometric method. The potency of scavenging activity following the addition of authentic ONOO- was in the following order: witch hazel bark > rosemary > jasmine tea > sage > slippery elm > black walnut leaf > Queen Anne's lace > Linden flower. The extracts exhibited dose-dependent ONOO- scavenging activities. We found that witch hazel (Hamamelis virginiana L.) bark showed the strongest effect for scavenging ONOO- of the 28 herbs. Hamamelitannin, the major active component of witch hazel bark, was shown to have a strong ability to scavenge ONOO-. It is suggested that hamamelitannin might be developed as an effective peroxynitrite scavenger for the prevention of ONOO- involved diseases. Copyright (C) 2002 John Wiley Sons, Ltd.