Induction by carrageenan inflammation of prepronociceptin mRNA in VR1-immunoreactive neurons in rat dorsal root ganglia
Induction by carrageenan inflammation of prepronociceptin mRNA in VR1-immunoreactive neurons in rat dorsal root ganglia
复制标题
DOI：
10.1016/s0168-0102(01)00230-9
复制
发表时间：
2001-07-01
影响因子：
2.9
通讯作者：
Kuraishi, Y
中科院分区：
文献类型：
--
作者：
Itoh, M;Takasaki, I;Kuraishi, Y
孤啡肽（Nociceptin，Nociceptin FQ）作用于初级传入神经，参与伤害性信息加工的调节。我们已经证明了。采用逆转录-聚合酶链反应（RT-PCR）技术，研究了角叉菜胶引起的外周炎症反应诱导背根神经节（DRG）前原感受肽（PPN）mRNA表达的变化。本实验采用原位杂交技术，研究外周炎症后初级感觉神经元中PPN mRNA的定位。足底注射角叉菜胶可诱导背根节中小神经元PPN mRNA的表达，角叉菜胶作用0.5h达高峰，6 h后减弱。所有PPN mRNA阳性神经元均呈香草酸受体亚型1（VR 1）阳性，部分VR 1阳性神经元PPN mRNA阴性。结果表明，外周炎症诱导VR 1阳性初级感觉神经元的亚群中产生伤害感受素，并支持伤害感受素在那里产生参与伤害性处理的调节的想法。(C)2001 Elsevier Science爱尔兰有限公司和日本神经科学学会。All rights reserved.
Nociceptin (orphanin FQ) may act on primary afferents and be involved in the regulation of nociceptive processing. We have shown. using reverse transcription-polymerase chain reaction (RT-PCR), that carrageenan-produced peripheral inflammation induces the expression of prepronociceptin (PPN) mRNA in the dorsal root ganglia (DRG). The present experiments were conducted to determine the localization of PPN mRNA in primary sensory neurons after peripheral inflammation, using in situ hybridization. An intraplantar injection of carrageenan induced the expression of PPN mRNA in small and medium sized neurons in the DRG: the effect peaked 0.5 h after carrageenan and subsided by 6 h. All neurons positive for PPN mRNA were positive for vanilloid receptor subtype 1 (VR1)-like immunoreactivity and some VR1-immunoreactive neurons were negative for PPN mRNA. The results suggest that peripheral inflammation induces the production of nociceptin in a sub-population of VR1-positive primary sensory neurons and support the idea that nociceptin produced there is involved in the regulation of nociceptive processing. (C) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.