A novel unsaturated fatty acid hydratase toward C16 to C22 fatty acids from Lactobacillus acidophilus
A novel unsaturated fatty acid hydratase toward C16 to C22 fatty acids from Lactobacillus acidophilus
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DOI：
10.1194/jlr.m059444
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发表时间：
2015-07-01
影响因子：
6.5
通讯作者：
Ogawa, Jun
中科院分区：
文献类型：
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作者：
Hirata, Akiko;Kishino, Shigenobu;Ogawa, Jun
羟基FAs是PUFAs的肠道微生物代谢产物之一，因其具有多种生物活性而备受关注。本研究的目的是鉴定具有将亚油酸（LA）转化为羟基FAs能力的乳酸菌。筛选过程表明，一种肠道细菌嗜酸乳杆菌NTV001可将LA主要转化为13-羟基顺式-9-十八烯酸，并鉴定出其水解酶脂肪酸水解酶1 （FA-HY1）。纯化了重组FA-HY1，并对其酶学特性进行了研究。FA-HY1不仅能转化C18 PUFAs，还能转化C20和C22 PUFAs。在δ 12位置具有顺式碳-碳双键的C18 PUFAs转化为相应的13-羟基FAs。花生四烯酸和DHA分别转化为相应的15-羟基FA和14-羟基FA。据我们所知，这是第一次报道细菌FA水合酶可以将C20和C22 PUFAs转化为相应的羟基FAs。这些利用FA-HY1产生的新型羟基FAs有助于阐明羟基FAs的生物活性。
Hydroxy FAs, one of the gut microbial metabolites of PUFAs, have attracted much attention because of their various bioactivities. The purpose of this study was to identify lactic acid bacteria with the ability to convert linoleic acid (LA) to hydroxy FAs. A screening process revealed that a gut bacterium, Lactobacillus acidophilus NTV001, converts LA mainly into 13-hydroxy-cis -9-octadecenoic acid and resulted in the identification of the hydratase responsible, fatty acid hydratase 1 (FA-HY1). Recombinant FA-HY1 was purified, and its enzymatic characteristics were investigated. FA-HY1 could convert not only C18 PUFAs but also C20 and C22 PUFAs. C18 PUFAs with a cis carbon-carbon double bond at the Delta 12 position were converted into the corresponding 13-hydroxy FAs. Arachidonic acid and DHA were converted into the corresponding 15-hydroxy FA and 14-hydroxy FA, respectively. To the best of our knowledge, this is the first report of a bacterial FA hydratase that can convert C20 and C22 PUFAs into the corresponding hydroxy FAs. These novel hydroxy FAs produced by using FA-HY1 should contribute to elucidating the bioactivities of hydroxy FAs.