Differential effects of Pax3 on expression of polysialyltransferases STX and PST in TGF-β-treated normal murine mammary gland cells
Differential effects of Pax3 on expression of polysialyltransferases STX and PST in TGF-β-treated normal murine mammary gland cells
复制标题
Pax3 对 TGF-β 处理的正常鼠乳腺细胞中聚唾液酸转移酶 STX 和 PST 表达的差异影响
DOI：
10.1177/1535370216669838
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发表时间：
2017-01-01
影响因子：
3.2
通讯作者：
Guan, Feng
中科院分区：
文献类型：
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作者：
Guo, Dong;Guo, Jia;Guan, Feng
哺乳动物细胞表面某些蛋白质的糖基化是致癌过程中的一个重要事件。唾液酸化是糖基化的一种类型，可以作用于多种细胞行为，例如迁移、生长和恶性侵袭。两种聚唾液酸转移酶 ST8Sia II (STX) 和 ST8Sia IV (PST) 负责神经细胞粘附分子上聚唾液酸的合成。我们之前表明，STX 和 PST 在经历转化生长因子-b 诱导的上皮间质转化的正常小鼠乳腺细胞中相反表达。 STX 和 PST 调节的分子基础仍不清楚。在本研究中，我们观察到转录因子Pax3上调STX表达，下调PST表达，并上调PSA表达，PSA主要附着于神经细胞粘附分子形成PSA-NCAM。正常小鼠乳腺细胞中Pax3的过表达改变了上皮-间质转化标志物E-钙粘蛋白和N-钙粘蛋白的表达，并显着促进细胞迁移，但对细胞增殖没有影响。
Glycosylation of certain proteins at the mammalian cell surface is an essential event in carcinogenesis. Sialylation, one type of glycosylation, can act on multiple cell-behaviors, such as migration, growth, and malignant invasion. Two polysialyltransferases, ST8Sia II (STX) and ST8Sia IV (PST), are responsible for synthesis of polysialic acid on neural cell adhesion molecule. We showed previously that STX and PST are oppositely expressed in normal murine mammary gland cells undergoing transforming growth factor-b-induced epithelial-mesenchymal transition. The molecular basis for regulation of STX and PST remained unclear. In the present study, we observed that transcription factor Pax3 upregulates STX expression, downregulates PST expression, and modulates upregulated expression of PSA, which attaches primarily to neural cell adhesion molecule to form PSA-NCAM. Overexpression of Pax3 in normal murine mammary gland cells transformed the expression of epithelial- mesenchymal transition markers E-cadherin and N-cadherin, and significantly promoted cell migration, but had no effect on cell proliferation.