Distribution of Th17 cells and Foxp3-expressing T cells in tumor-infiltrating lymphocytes in patients with uterine cervical cancer
Distribution of Th17 cells and Foxp3-expressing T cells in tumor-infiltrating lymphocytes in patients with uterine cervical cancer
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宫颈癌患者肿瘤浸润淋巴细胞中Th17细胞和Foxp3表达T细胞的分布
DOI：
10.1016/j.cca.2012.07.012
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发表时间：
2012-11-20
影响因子：
5
通讯作者：
Cui, Baoxia
中科院分区：
文献类型：
--
作者：
Hou, Fei;Li, Zhen;Cui, Baoxia
背景：最近的研究表明Th17细胞对肿瘤有潜在的影响。在本研究中，我们研究了Th17细胞与foxp3表达T细胞在宫颈癌（UCC）患者、宫颈上皮内瘤变（ON）患者和健康宫颈组织的肿瘤浸润淋巴细胞（Ills）中的分布。方法：采用免疫组化染色法检测Th17细胞和表达foxp3的T细胞。采用酶联免疫吸附试验（ELISA）检测IL-6、tgf - β、IL-17和IL-10。免疫组织化学染色微血管密度（MVD）以评估IL-17表达与血管生成的关系。结果：与对照组相比，UCC或CIN患者Th17细胞和表达foxp3的T细胞比例较高，当til中Th17/ foxp3表达的T细胞比例在个别病例中降低时，til中Th17/ foxp3表达的T细胞比例比正常宫颈组织明显降低。同时，细胞因子（IL-6、tgf - β和IL-10）浓度在UCC患者中显著高于健康对照组。有趣的是，肿瘤内Th17细胞的水平与MVD呈正相关。结论：Th17/ foxp3表达的T细胞失衡可能在UCC的发生发展中起关键作用，Th17细胞可能通过促进血管生成促进肿瘤进展。(C) 2012 Elsevier B.V.版权所有
Background: Recent studies suggest a potential impact of Th17 cells on tumor. In the present study, we investigated the distribution of Th17 cells in relation to Foxp3-expressing T cells in the tumor-infiltrating lymphocytes (Ills) from patients with uterine cervical cancer (UCC), cervical tissues from patients with cervical intraepithelial neoplasia (ON) and healthy cervical tissues.Methods: Th17 cells and Foxp3-expressing T cells were evaluated by immunohistochemical staining. IL-6, TGF-beta, IL-17 and IL-10 were detected by enzyme-linked immunosorbent assay (ELISA). Immunohistochemical staining for microvessel density (MVD) was performed in order to assess the association of IL-17 expression with angiogenesis.Results: Compared with controls, patients with UCC or CIN had a higher proportion of Th17 cells and Foxp3-expressing T cells, when the ratio of Th17/Foxp3-expressing T cells in TILs was decreased in individual cases, it was more markedly decreased in TILs than normal cervical tissues. Meanwhile, the cytokine(IL-6, TGF-beta and IL-10) concentrations were significantly higher in UCC patients than those in healthy controls. Interestingly, the levels of intratumoral Th17 cells were positively correlated with MVD in tumors.Conclusions: The imbalance of Th17/Foxp3-expressing T cells may play critical roles in the development and progression of UCC and Th17 cells may promote tumor progression by fostering angiogenesis. (C) 2012 Elsevier B.V. All rights reserved.