Compensation-free, all-fiber-optic, two-photon endomicroscopy at 1.55 μm.
Compensation-free, all-fiber-optic, two-photon endomicroscopy at 1.55 μm.
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DOI：
10.1364/ol.36.001299
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发表时间：
2011-04-01
期刊：
影响因子：
3.6
通讯作者：
Li X
中科院分区：
文献类型：
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作者：
Murari K;Zhang Y;Li S;Chen Y;Li MJ;Li X
我们提出了一种具有 1.55 μm 激发的全光纤扫描多光子内窥镜，无需在内窥镜之前预啁啾飞秒脉冲。该系统由 1.55 μm 飞秒光纤激光器、用于光传输和荧光收集的定制双包层光纤以及压电扫描头组成。我们展示了培养细胞和小鼠组织的双光子成像，两者均用吲哚菁绿标记。具有近红外发射的自由空间多光子成像先前已显示出减少背景荧光和降低荧光发射衰减的优点。对于光纤多光子成像，在光纤中使用电信波长（1.3-1.6 μm）的孤子效应还有一个额外的优点，即允许无需色散补偿的小型系统。我们预计这些优势将有助于将多光子内窥镜技术过渡到临床。
We present an all-fiber-optic scanning multiphoton endomicroscope with 1.55 μm excitation without the need for prechirping femtosecond pulses before the endomicroscope. The system consists of a 1.55 μm femtosecond fiber laser, a customized double-clad fiber for light delivery and fluorescence collection, and a piezoelectric scan head. We demonstrate two-photon imaging of cultured cells and mouse tissue, both labeled with indocyanine green. Free-space multiphoton imaging with near-IR emission has previously shown benefits in reduced background fluorescence and lower attenuation for the fluorescence emission. For fiber-optic multiphoton imaging there is the additional advantage of using the soliton effect at the telecommunication wavelengths (1.3–1.6 μm) in fibers, permitting dispersion-compensation-free, small-footprint systems. We expect these advantages will help transition multiphoton endomicroscopy to the clinic.