Expression and localization of opioid receptors during the maturation of human oocytes
Expression and localization of opioid receptors during the maturation of human oocytes
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DOI：
10.1016/j.rbmo.2012.02.007
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发表时间：
2012-05-01
影响因子：
4
通讯作者：
Agirregoitia, Naiara
中科院分区：
文献类型：
--
作者：
Agirregoitia, Ekaitz;Peralta, Laura;Agirregoitia, Naiara
内源性阿片受体系统在女性生殖器官中有一定的表达，但其在人类卵母细胞中的表达却知之甚少。本研究探讨了人卵母细胞成熟过程中阿片受体的存在和差异分布。本研究共收集了821个人卵母细胞进行卵胞浆内单精子注射（ICSI），其中213个处于生殖泡（GV）期，164个处于中期I（MI）期，444个受精失败的中期II（MII）卵母细胞。此外，还纳入了31个MII卵母细胞（对应于未尝试ICSI的病例）和50个IVF计划中受精失败的MII卵母细胞。蛋白质印迹分析揭示了人卵母细胞中δ（OPRD 1）、κ（OPRK 1）和μ（OPRM 1）阿片受体的存在。在卵母细胞成熟过程中，OPRK1和OPRM 1的免疫染色模式发生了变化，而OPRD 1的模式始终是相同的。特别地，在GV至MII期的外周组织中检测到OPRD 1。OPRK1在GV期外周分布，MI期更多在内部，MII期均匀分布。最后，OPRM 1位于外周的GV期和均匀的MI和MII卵母细胞。阿片类物质可能通过受体在卵母细胞成熟中发挥作用。(C)2012年，爱思唯尔有限公司出版的生殖保健有限公司。版权所有。
The endogenous opioid system has been characterized in some female reproductive organs, but little is known about the expression of these receptors in human oocytes. This study investigated the presence and differential distribution of the opioid receptors during the maturation of human oocytes. A total of 821 human oocytes from an intracytoplasmic sperm injection (ICSI) programme were studied including 213 at germinal-vesicle (GV) stage and 164 at metaphase-I (MI) stage and 444 failed fertilization metaphase-II (MII) oocytes. Additionally 31 MII oocytes corresponding to cases where ICSI was not attempted and 50 failed fertilization MII oocytes from the IVF programme were included. Western blot analysis revealed the presence of the delta (OPRD1), kappa (OPRK1) and mu (OPRM1) opioid receptors in human oocytes. The OPRK1 and OPRM1 immunostaining patterns changed during the maturation of the oocyte, while the OPRD1 pattern was the same throughout. In particular, OPRD1 were detected in peripheral tissue from the GV to the MII stage. OPRK1 were found peripherally at the GV stage, more internally at MI and homogeneously at MII. Finally, OPRM1 were located peripherally at the GV stage and homogeneously in MI and MII oocytes. Opioids may have a role in oocyte maturation, acting via receptors. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.