Cysteine-reactive polymers synthesized by atom transfer radical polymerization for conjugation to proteins
Cysteine-reactive polymers synthesized by atom transfer radical polymerization for conjugation to proteins
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DOI：
10.1021/ja045063m
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发表时间：
2004-12-01
影响因子：
15
通讯作者：
Maynard, HD
中科院分区：
文献类型：
--
作者：
Bontempo, D;Heredia, KL;Maynard, HD
在这篇文章中，我们报道了一种合成半远螯聚合物的策略。制备了一种吡啶基二硫化物修饰的引发剂，并将其用于CuBr/2，2 '-联吡啶介导的甲基丙烯酸羟乙酯的原子转移自由基聚合（ATRP）。不同分子量的聚合物的多分散性指数（Mw/Mn）为1.25或更小。在聚合过程中，吡啶基二硫化物端基被保留，并允许聚合物与牛血清白蛋白的半胱氨酸残基直接缀合。所描述的方法提供了一种通过可逆二硫键制备蛋白质-聚合物缀合物的一般方法，而不需要对聚合物进行合成后修饰。
In this communication we report a strategy for the synthesis of semitelechelic polymers reactive to cysteines. An initiator modified with a pyridyl disulfide was prepared and used for the CuBr/2,2‘-bipyridine-mediated atom transfer radical polymerization (ATRP) of 2-hydroxyethyl methacrylate. Polydispersity indices (Mw/Mn) of the polymers with different molecular weights were 1.25 or less. The pyridyl disulfide end group was preserved during the polymerization and allowed direct conjugation of the polymer to cysteine residues of bovine serum albumin. The described method provides a general way for the preparation of protein−polymer conjugates through a reversible disulfide bond without the need for postsynthesis modification of the polymers.