A SYNTHETIC ANALOG OF VITAMIN-D3, 22-OXA-1,25-DIHYDROXY-VITAMIN-D3, STIMULATES THE PRODUCTION OF PROSTACYCLIN BY VASCULAR TISSUES
A SYNTHETIC ANALOG OF VITAMIN-D3, 22-OXA-1,25-DIHYDROXY-VITAMIN-D3, STIMULATES THE PRODUCTION OF PROSTACYCLIN BY VASCULAR TISSUES
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DOI：
10.1016/0024-3205(92)90511-m
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发表时间：
1992-01-01
期刊：
影响因子：
6.1
通讯作者：
ONAYA, T
中科院分区：
文献类型：
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作者：
INOUE, M;WAKASUGI, M;ONAYA, T
我们研究了22-oxa-1，25-dihydroxyvitamin D3（一种维生素D3的合成类似物）对使用大鼠主动脉环和来自胎鼠主动脉平滑肌的A7 r5细胞的血管组织产生前列环素的影响。用22-oxa-1，25-dihydroxyvitamin D3处理的大鼠的主动脉环的前列环素合成比未处理的对照组高得多，但没有引起任何显著的高钙血症。用22-氧杂-1，25-二羟基维生素D3处理48小时，以剂量依赖性方式显著增加A7 r5细胞的前列环素产生。在时程研究中，与22-氧杂-1，25-二羟基维生素D3或1，25-二羟基维生素D3孵育的细胞在48小时内逐渐产生前列环素。与对照培养物相比，产生大量前列环素的最短孵育时间为24小时。我们观察到22-oxa-1，25-dihydroxyvitamin D3处理诱导A7 r5细胞中的环氧合酶mRNA。我们的数据表明，22-oxa-1，25-dihydroxyvitamin D3可能是一种保护物质，通过调节前列腺素代谢，防止动脉粥样硬化的发展。
We investigated the effect of 22-oxa- 1,25-dihydroxyvitamin D3, a synthetic analogue of vitamin D3, on the production of prostacyclin by vascular tissues using rat aortic rings and A7r5 cells derived from fetal rat aortic smooth muscle. Prostacyclin synthesis by aortic rings of rats treated with 22-oxa-1,25-dihydroxyvitamin D3 was much higher than that of non-treated controls, but did not cause any significant hypercalcemia. Treatment with 22-oxa- 1,25-dihydroxyvitamin D3 significantly increased the production of prostacyclin by A7r5 cells for 48 hours in a dose-dependent manner. In time-course studies, cells incubated with 22-oxa-1,25-dihydroxyvitamin D3 or 1,25-dihydroxyvitamin D3 produced prostacyclin progressively over a period of 48 hours. The shortest period of incubation that produced a significant amount of prostacyclin compared with control cultures was 24 hours. We observed that treatment with 22-oxa-1,25-dihydroxyvitamin D3 induced cyclooxygenase mRNA in A7r5 cells. Our data suggest that 22-oxa-1,25-dihydroxyvitamin D3 may possibly be a protective substance against the development of atherosclerosis by modulating prostaglandin metabolism.