Study on the mechanism of anti-MIRI action of total flavones of Fructus Chorspondiatis by PET/CT imaging.
Study on the mechanism of anti-MIRI action of total flavones of Fructus Chorspondiatis by PET/CT imaging.
复制标题
PET/CT显像研究紫藤总黄酮抗MIRI作用机制
DOI：
10.21037/cdt-20-305
复制
发表时间：
2020
影响因子：
2.4
通讯作者：
Jia
中科院分区：
文献类型：
--
作者：
Guo;Li;Hai;Yun;Wenrui Wang;Yulin He;Xue;Jia
背景
用~(13)N-氨水微区PET/CT显像等方法，研究柴胡总黄酮(TFFC)抗大鼠心肌缺血再灌注损伤(MIRI)的作用。
方法
雄性SD大鼠随机分为6组。造模前后行Micro PET/CT显像，计算心肌缺血区的体积(VOI)和平均舒张期(SUV)。检测氧化应激指数[超氧化物歧化酶(SOD)、丙二醛(MDA)]和心肌损伤标志酶[肌酸激酶(CK)、乳酸脱氢酶(LDH)]。HE染色观察心肌病理变化。建立大鼠体外心肌细胞MIRI模型，观察各组大鼠心肌细胞损伤和凋亡情况，并检测心肌细胞凋亡率。
结果
分别于24、48h观察显像剂的成像粘度。24 h的VOI分别为(6.33±2.02)、(6.01±1.56)和(3.32±0.86)mm3。48h的VOI分别为(3.31±1.33)、(2.61±1.01)和(1.32±0.58)mm3。72h成像中剂量组和高剂量组恢复正常，低剂量组仍稀疏，VOI为(1.26±0.68)mm~3。缺血区(SUV均值)随时间逐渐增加。代谢逐渐恢复(F=121.82,450.82,435.75)。三种剂量的TFFC均能清除自由基，减轻心肌损伤。其中，高剂量组对超氧化物歧化酶的影响较好，中剂量组对丙二醛和乳酸脱氢酶的影响较好。低剂量组对CK有影响，与对照组比较差异有统计学意义(P<；0.05)。给药后，各剂量组心肌细胞形态均有不同程度的改善。核固缩、破裂、凋亡率等明显降低，细胞数量增多。高剂量组改善最为明显。
结论
PET/CT成像方法可检测无创性、活体和动态的MIRI，并能准确评价蒙药TFFC对MIRI的保护作用。TFFC抗MIRI具有清除自由基、减轻氧化应激损伤、抑制细胞凋亡、影响相关酶活性等作用。
Background
To investigate anti myocardial ischemia/reperfusion injury (MIRI) action of total flavones of Fructus Chorspondiatis (TFFC) in rats by 13N-ammonia micro PET/CT imaging, etc.
Methods
Male Sprague-Dawley rats were randomly divided into 6 groups. Micro PET/CT imaging was performed before and after modeling to calculate the volume (VOI) and SUVmean of myocardial ischemic area. The oxidative stress index [(superoxide dismutase (SOD), malondialdehyde (MDA)] and the marker enzymes [creatine kinase (CK), lactate dehydrogenase (LDH)] of myocardial injury were detected. The pathological changes of myocardial were observed via HE staining. A MIRI model of rat cardiomyocytes in vitro was established, the damage and apoptosis of myocardial cells in each group were observed, and the apoptosis rate of cardiomyocytes was detected.
Results
The imaging viscosities of the imaging agents were observed at 24 and 48 h in each group. The VOI of 24 h imaging was (6.33±2.02), (6.01±1.56) and (3.32±0.86) mm3, respectively. The VOI of 48 h imaging was (3.31±1.33), (2.61±1.01) and (1.32±0.58) mm3. The 72 h imaging medium and high dose group recovered, while the low dose group still saw sparseness with (1.26±0.68) mm3 VOI. The ischemic (SUVmean) gradually increased with time. Metabolism gradually recovered (F=121.82, 450.82, 435.75, P<0.05). The three doses of TFFC can eliminate free radicals and reduce the damage of myocardial injury. Amongst them, the high-dose group had a better effect on SOD, and the middle-dose group had a better effect on MDA and LDH. The low-dose group affected CK, and a significant difference was observed compared with the control group (P<0.05). After administration, the morphology of myocardial cells in each dose group was improved to some extent. Nuclear pyknosis, rupture, the apoptosis rate, etc. were significantly reduced, the number of cells increased. The high dose group showed the most obvious improvement.
Conclusions
The PET/CT imaging method can detect non-invasive, in vivo and dynamic MIRI, and can accurately evaluate the protective effect of traditional Mongolian medicine TFFC on MIRI. The Anti-MIRI of TFFC can scavenge free radicals, reduce oxidative stress damage, inhibit apoptosis, affect the activity of related enzymes.
影响因子：
2.5
作者：
Arun K. Thukkani;K. Shoghi;Dong Zhou;Jinbin Xu;W. Chu;E. Novak;Delphine L. Chen;R. Gropler;R. Mach
通讯作者：
Arun K. Thukkani;K. Shoghi;Dong Zhou;Jinbin Xu;W. Chu;E. Novak;Delphine L. Chen;R. Gropler;R. Mach