Neutral metalloendopeptidase in human lung tissue and cultured cells.
Neutral metalloendopeptidase in human lung tissue and cultured cells.
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人肺组织和培养细胞中的中性金属内肽酶。
DOI：
10.1164/arrd.1985.132.3.564
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Erdos,EG
中科院分区：
文献类型：
--
作者：
Johnson,AR;Ashton,J;Schulz,WW;Erdos,EG
中性金属内肽酶（NEP）或“脑啡肽酶”在人肺组织和培养细胞中的分布与血管紧张素I转换酶（ACE）的分布进行了比较。在胎肺组织匀浆和离体气道和肺血管中测定NEP和ACE的比活性。NEP活性在气道组织中最高，ACE活性在离体血管中最高。人脐静脉和肺动脉内皮细胞均具有较高的ACE活性（80 ~ 90 nmol/h/106 cells），但仅有微量的NEP活性（0.5 ~ 0.6 nmol/h/106 cells）。从人肺培养的成纤维细胞ACE低，但丰富的NEP比培养的内皮细胞。人包皮或剖腹产皮肤的成纤维细胞是NEP活性最丰富的来源（60 ~ 80 nmol/h/106 cells）。免疫组织化学研究证实了生化分析。正如预期的那样，ACE定位于血管的管腔表面，其分布与内皮标记物因子VIII抗原的分布相似。相反，NEP位于肺泡隔内。与培养的成纤维细胞相比，培养的内皮细胞对NEP仅弱染色。这2种酶在不同细胞中的位置和肽底物特异性的差异表明，它们依次作用于循环肽或微血管床内释放的肽。
The distribution of a neutral metalloendopeptidase (NEP), or “enkephalinase,” in human lung tissue and cultured cells was compared with that of angiotensin I converting enzyme (ACE). The specific activities of NEP and ACE were measured in homogenates of fetal lung tissue and in isolated airways and pulmonary vessels. NEP activity was highest in airway tissue, and ACE activity was highest in isolated vessels. Human endothelial cells from either umbilical veins or pulmonary arteries had high ACE activity (80 to 90 nmol/h/106cells) but only a trace of NEP activity (0.5 to 0.6 nmol/h/106cells). Fibroblasts cultured from human lungs were low in ACE but richer in NEP than cultured endothelial cells. Fibroblasts from human foreskins or caesarean section skin were the richest source of NEP activity (60 to 80 nmol/h/106cells). Immunohistochemical studies confirmed the biochemical assays. As expected, ACE was localized on the luminal surface of blood vessels, with a distribution similar to that of factor VIII antigen, an endothelial marker. In contrast, NEP was localized within the alveolar septa. Cultured endothelial cells stained only weakly for NEP in contrast to cultured fibroblasts. The location of these 2 enzymes in different cells and the differences in peptide substrate specificity suggests that they act sequentially on circulating peptides or those released within microvascular beds.
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影响因子：
2.9
作者：
Weare,JA;Gafford,JT;Lu,HS;Erdös,EG
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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人类男性生殖道中的中性金属内肽酶。
DOI：
--
发表时间：
1985
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Erdos,EG;Schulz,WW;Gafford,JT;Defendini,R
通讯作者：
Defendini,R
影响因子：
4.1
作者：
FULCHER, IS;KENNY, AJ
通讯作者：
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