TcRho1 of Trypanosoma cruzi: role in metacyclogenesis and cellular localization.
TcRho1 of Trypanosoma cruzi: role in metacyclogenesis and cellular localization.
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DOI：
10.1016/j.bbrc.2004.08.197
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发表时间：
2004-10
影响因子：
3.1
通讯作者：
L. D. B. de Melo;J. L. Nepomuceno-Silva;C. Sant’Anna;N. Eisele;Rodrigo B Ferraro;J. Meyer‐Fernandes;W. de Souza;N. Cunha-e-Silva;U. Lopes
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文献类型：
--
作者：
L. D. B. de Melo;J. L. Nepomuceno-Silva;C. Sant’Anna;N. Eisele;Rodrigo B Ferraro;J. Meyer‐Fernandes;W. de Souza;N. Cunha-e-Silva;U. Lopes
Here we have investigated the function of TcRho1, a Rho family orthologue from the parasite Trypanosoma cruzi. We have selected parasites overexpressing wild-type TcRho1 and a truncated form of TcRho1 (TcRho1-ΔCaaX) which is unable to undergo farnesylation and supposed to interfere with recruitment of Rho effectors to membranes. TcRho1 protein was localized at the anterior region of wild-type and TcRho1 overexpressing epimastigotes, suggesting association with the Golgi apparatus. Accordingly, parasites overexpressing TcRho1-ΔCaaX presented cytoplasmic fluorescence. To address the function of TcRho1 during differentiation, from epimastigotes to trypomastigotes, we submitted parasites overexpressing the above-cited lineages to metacyclogenesis assays. Parasites overexpressing TcRho1-ΔCaaX generated a discrete number of metacyclic trypomastigotes when compared with other lineages. Strikingly, TcRho1-ΔCaaX cells died synchronously during the process of metacyclogenesis.
