Harnessing the versatility of PLGA nanoparticles for targeted Cre-mediated recombination.
Harnessing the versatility of PLGA nanoparticles for targeted Cre-mediated recombination.
复制标题
利用 PLGA 纳米粒子的多功能性进行 Cre 介导的靶向重组。
DOI：
10.1016/j.nano.2019.02.027
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Ardehali,Reza
中科院分区：
文献类型：
--
作者：
Nguyen,NgocB;Chen,Cheng-Han;Zhang,Yulong;Zhao,Peng;Wu,BenjaminM;Ardehali,Reza
Ligand-dependent Cre recombinases are pivotal tools for the generation of inducible somatic mutants. This method enables spatial and temporal control of gene activity through tamoxifen administration, providing new avenues for studying gene function and establishing animal models of human diseases. While this paved the way for developmental studies previously deemed impractical, the generation of tissue-specific transgenic mouse lines can be time-consuming and costly. Herein, we design a ‘smart’, biocompatible, and biodegradable nanoparticle system encapsulated with tamoxifen that is actively targeted to specific cell typesin vivothrough surface conjugation of antibodies. We demonstrate that these nanoparticles bind to cells of interest and activate Cre recombinase, resulting in tissue-specific Cre activation. This system provides a versatile, yet powerful approach to induce recombination in a ubiquitious Cre system for various biomedical applications and sets the stage for a time- and cost-effective strategy of generating new transgenic mouse lines.
