Synthesis of surface nano-molecularly imprinted polymers for sensitive baicalin detection from biological samples.
Synthesis of surface nano-molecularly imprinted polymers for sensitive baicalin detection from biological samples.
复制标题
表面纳米分子印迹聚合物的合成，用于生物样品中黄芩苷的灵敏检测
DOI：
10.1039/c5ra04424c
复制
发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Yuan CS
中科院分区：
文献类型：
--
作者：
Gu X;He H;Wang CZ;Gao Y;Zhang H;Hong J;Du S;Chen L;Yuan CS
制备了表面分子印迹聚合物（MIP@SBA-15），用于生物样品中黄芩苷（BA）的选择性提取和检测。采用傅里叶变换红外（FT-IR）、扫描电镜（SEM）、透射电镜（TEM）、热重分析（TGA）和氮吸附-脱附等温线对印迹聚合物和非印迹聚合物的表面形貌和特征进行了表征。结果表明，聚合物成功接枝在SBA-15表面，具有高度有序的介孔结构。结合实验中MIP@SBA-15在80 min内达到饱和吸附，对吸附量大的BA有明显的特异性识别。同时，将制备的MIP@SBA-15作为生物样品固相萃取BA的选择性吸附剂。肝脏和脾脏BA的加样回收率为90.6% ~ 90.9%,RSD < 3.7%。结果表明，该方法简便、快速、灵敏，可有效提取和检测生物样品中的痕量BA。
Surface molecularly imprinted polymers (MIP@SBA-15) imprinted on the surface of hybrid nanostructured organic/inorganic materials (SBA-15) were prepared for the selective extraction and detection of baicalin (BA) from biological samples. The surface morphologies and characteristics of the imprinted and non-imprinted polymers were characterized by Fourier transform infrared (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermo–gravimetric analysis (TGA) and nitrogen adsorption–desorption isotherms. The results indicated that the polymers were successfully grafted on the surface of SBA-15 and possessed a highly ordered mesoporous structure. In binding tests, MIP@SBA-15 reached saturated adsorption within 80 min and exhibited significant specific recognition toward BA with large adsorption capacity. Meanwhile, the prepared MIP@SBA-15 was used as a selective sorbent for solid-phase extraction of BA from biological samples. Recoveries of BA from the liver and spleen ranged from 90.6% to 90.9% with RSD < 3.7%. All these results reveal that this method is simple, rapid and sensitive for effectively extracting and detecting trace BA in biological samples.