MiR-30c-5p suppresses migration, invasion and epithelial to mesenchymal transition of gastric cancer via targeting MTA1
MiR-30c-5p suppresses migration, invasion and epithelial to mesenchymal transition of gastric cancer via targeting MTA1
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DOI：
10.1016/j.biopha.2017.06.084
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发表时间：
2017-09-01
影响因子：
7.5
通讯作者：
Huang, Kun-ming
中科院分区：
文献类型：
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作者：
Cao, Jing-mei;Li, Gao-zhong;Huang, Kun-ming
背景：胃癌（gastric cancer，GC）是我国常见的恶性肿瘤.近年来的研究表明，microRNA在肿瘤发生过程中起着重要作用。方法：采用RT-qPCR方法检测40例胃癌组织和5例胃癌细胞中miR-30 c-5 p及其靶蛋白的表达水平。同时对miR 30 c-5 p表达水平与临床病理特征进行相关性分析。另外，通过创伤愈合实验和细胞侵袭实验观察miR-30 c-5 p对胃癌细胞迁移和侵袭能力的影响。结果：胃癌组织中miR-30 c-5 p的表达水平明显低于正常对照组，且与肿瘤的临床分期、淋巴结转移密切相关。此外，miR-30 c-5 p的敲低可增强胃癌细胞的迁移和侵袭能力，而miR-30 c-5 p的过表达则可逆转胃癌细胞的迁移和侵袭能力。进一步的研究表明，miR-30 c-5 p抑制其靶点转移相关蛋白1（MTA 1）的表达，进而抑制上皮间质转化（EMT）过程，而EMT在胃癌的转移中起重要作用。结果表明，miR-30 c-5 p是一种新的肿瘤发生抑制因子，可通过MTA 1抑制肿瘤的转移和EMT，这可能为胃癌的治疗提供一个可能的靶点。(C)2017年Elsevier Masson SAS。All rights reserved.
Background: In China, gastric cancer (GC) is an ordinary malignant tumor. Recent literatures have shown that microRNA is critical during tumorigenesis. This study focuses on the influence of miR-30c-5p on the metastasis of GC and further explores its underlying mechanism.Methods: Before the study, expression level of miR-30c-5p and targeted protein was detected in 40 GC tissue samples and 5 GC cells by RT-qPCR. Meanwhile, correlation analysis was conducted between miR30c- 5p expression level and clinicopathological features. In addition, wound healing assay and cell invasion assay were utilized to identify whether miR-30c-5p could affect the migrated and invaded ability of GC cells. Western blotting assay and luciferase assay were used to explore the potential mechanism.Results: In GC tissues, miR-30c-5p expression level was significantly lower and was remarkably related with clinical features such as tumor node metastasis(TNM) stage and lymphatic metastasis. Moreover, the migrated and invaded ability of GC cells was enhanced through knockdown of miR-30c-5p, while overexpression of miR-30c-5p presented with reversed effect. Further study showed that miR-30c-5p inhibited the expression of its target spot, metastasis-associated protein 1(MTA1), and then suppressed the process of epithelial to mesenchymal transition(EMT) which was important in the metastasis of GC.Conclusion: The results indicate that miR-30c-5p, a novel suppressor in tumorigenesis, could inhibit the metastasis and EMT via MTA1, which may offer a possible therapeutic target in GC. (C) 2017 Elsevier Masson SAS. All rights reserved.