Small unilamellar vesicles containing glycophorin A. Chemical characterization and proton nuclear magnetic resonance studies.
Small unilamellar vesicles containing glycophorin A. Chemical characterization and proton nuclear magnetic resonance studies.
复制标题
含有血型糖蛋白 A 的小单层囊泡。化学表征和质子核磁共振研究。
DOI：
10.1021/bi00518a008
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Prestegard,JH
中科院分区：
文献类型：
--
作者：
Ong,RL;Marchesi,VT;Prestegard,JH
可以制备每20个脂质分子含有1个蛋白质分子的囊泡。通过使用水解酶，如神经氨酸酶和胰蛋白酶，结合氨基酸分析得到的囊泡上的数据表明，血型糖蛋白被纳入一个transbilayer的方式与高fractionof分子取向与含碳水化合物的氨基末端的囊泡外部。在质子核磁共振谱中，蛋白质与双层疏水部分的相互作用是明显的，并且脂质线宽增加已被用于表征相互作用的强度和化学计量。发现血型糖蛋白直接影响多达40个脂质分子/蛋白质分子;然而，影响的幅度并不大。膜中蛋白质和脂质成分的相互作用是许多膜中心现象的一个重要方面。已经采用了各种技术，并且研究了各种脂质-蛋白质系统，以努力阐明这些相互作用的分子细节[关于综述，参见Chapman et al.（1979）]。现在很明显，许多完整膜蛋白可能具有适当长度的疏水片段以跨越脂质双层
Vesicles with as much as 1 molecule of protein per20 molecules of lipid can be prepared. Data on the vesicles obtained by using hydrolytic enzymes such as neuraminidase and trypsin, combined with amino acid analysis, suggest that glycophorin is incorporated in a transbilayer fashion with a high fractionof the molecules oriented with the carbohydrate-containing amino terminus to the vesicle exterior. In-teraction of the protein with the hydrophobic portion of the bilayer is apparent in proton nuclear magnetic resonance spectra, and lipid line-width increases have been used to characterize the strength and stoichiometry of interaction. Glycophorin is found to affect directly as many as 40 lipid molecules per molecule of protein; however, the magnitude of the effects is not large. e interaction of protein and lipid constituents of mem-branes is an important aspect of many membrane-centered phenomena. A variety of techniques have been employed, and a variety of lipid-protein systems studied, in an effort to elucidate the molecular details of those interactions [for a review, see Chapman et al.(1979)]. It is now apparent that a number of integralmembrane proteins may have hydrophobic segments of appropriate length tospan a lipid bilayer