Lymphatic Pump Treatment Augments Lymphatic Flux of Lymphocytes in Rats
Lymphatic Pump Treatment Augments Lymphatic Flux of Lymphocytes in Rats
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DOI：
10.1089/lrb.2010.0009
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发表时间：
2010-01-01
影响因子：
1.4
通讯作者：
Hodge, Lisa M.
中科院分区：
文献类型：
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作者：
Huff, Jamie B.;Schander, Artur;Hodge, Lisa M.
背景资料：淋巴泵技术（LPT）被骨科医生用于治疗水肿和感染;然而，LPT增强淋巴和免疫系统的机制知之甚少。方法和结果：为了测量LPT对大鼠的影响，对10只大鼠的乳糜池（CC）进行插管，并在1）LPT前基线，2）LPT 4分钟，3）LPT后10 min恢复。LPT显著增加（p < 0.05）淋巴流量，从基线24 +/- 5 μ l/min增加到89 +/- 30 μ l/min。CC淋巴细胞流量基线为0.65 +/- 0.21 × 10（6）淋巴细胞/min，LPT将CC淋巴细胞流量增加到6.10 +/- 0.99 × 10（6）淋巴细胞/min（p < 0.01）。LPT对任何淋巴细胞群体均无优先效应，因为总淋巴细胞、CD 4 + T细胞、CD 8 + T细胞和B细胞数量均类似地增加。为了确定LPT是否动员肠相关淋巴细胞进入CC淋巴，通过对CC淋巴细胞进行肠归巢受体整合素α 4 β 7染色来鉴定CC淋巴中的肠相关淋巴细胞。LPT显著增加（p < 0.01）α 4 β 7阳性CC淋巴细胞的流量，从基线的0.70 +/- 0.03 × 10（5）淋巴细胞/min增加到LPT期间的6.50 +/- 0.10 × 10（5）淋巴细胞/min。最后，淋巴细胞通量在恢复过程中是类似的基线，表明LPT的影响是transient.Conclusions：总的来说，这些结果表明，LPT可能会提高免疫监视通过增加淋巴细胞释放到淋巴循环，特别是从肠道相关的淋巴组织的数量。大鼠为进一步研究LPT对淋巴和免疫系统的影响提供了有用的模型。
Background: Lymphatic pump techniques (LPT) are used by osteopathic practitioners for the treatment of edema and infection; however, the mechanisms by which LPT enhances the lymphatic and immune systems are poorly understood.Methods and Results: To measure the effect of LPT on the rat, the cisterna chyli (CC) of 10 rats were cannulated and lymph was collected during 4 min of 1) pre-LPT baseline, 2) 4 min LPT, and 3) 10 min post-LPT recovery. LPT increased significantly (p < 0.05) lymph flow from a baseline of 24 +/- 5 mu l/min to 89 +/- 30 mu l/min. The baseline CC lymphocyte flux was 0.65 +/- 0.21 x 10(6) lymphocytes/min, and LPT increased CC lymphocyte flux to 6.10 +/- 0.99 x 10(6) lymphocytes/min (p < 0.01). LPT had no preferential effect on any lymphocyte population, since total lymphocytes, CD4+ T cells, CD8+ T cells, and B cell numbers were similarly increased. To determine if LPT mobilized gut-associated lymphocytes into the CC lymph, gut-associated lymphocytes in the CC lymph were identified by staining CC lymphocytes for the gut homing receptor integrin alpha 4 beta 7. LPT significantly increased (p < 0.01) the flux of alpha 4 beta 7 positive CC lymphocytes from a baseline of 0.70 +/- 0.03 x 10(5) lymphocytes/min to 6.50 +/- 0.10 x 10(5) lymphocytes/min during LPT. Finally, lymphocyte flux during recovery was similar to baseline, indicating the effects of LPT are transient.Conclusions: Collectively, these results suggest that LPT may enhance immune surveillance by increasing the numbers of lymphocytes released in to lymphatic circulation, especially from the gut associated lymphoid tissue. The rat provides a useful model to further investigate the effect of LPT on the lymphatic and immune systems.