CIRCADIAN OSCILLATIONS OF CYTOSOLIC AND CHLOROPLASTIC FREE CALCIUM IN PLANTS
CIRCADIAN OSCILLATIONS OF CYTOSOLIC AND CHLOROPLASTIC FREE CALCIUM IN PLANTS
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DOI：
10.1126/science.7569925
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发表时间：
1995-09-29
期刊：
影响因子：
56.9
通讯作者：
TREWAVAS, A
中科院分区：
文献类型：
--
作者：
JOHNSON, CH;KNIGHT, MR;TREWAVAS, A
烟草和拟南芥植物，表达转基因的钙敏感的发光蛋白脱辅基水母发光蛋白，揭示了昼夜节律振荡的游离胞质钙，可以通过光暗信号相移。当脱辅基水母发光蛋白的叶绿体，昼夜叶绿体钙节奏同样观察到转移后的幼苗恒定黑暗。预计游离钙浓度的昼夜节律振荡将控制许多钙依赖性酶和过程，从而影响昼夜节律的输出。因此，钙流的调节是昼夜节律系统组织的基础。
Tobacco and Arabidopsis plants, expressing a transgene for the calcium-sensitive luminescent protein apoaequorin, revealed circadian oscillations in free cytosolic calcium that can be phase-shifted by light-dark signals. When apoaequorin was targeted to the chloroplast, circadian chloroplast calcium rhythms were likewise observed after transfer of the seedlings to constant darkness. Circadian oscillations in free calcium concentrations can be expected to control many calcium-dependent enzymes and processes accounting for circadian outputs. Regulation of calcium flux is therefore fundamental to the organization of circadian systems.