Label free bio image sensor for real time monitoring of potassium ion released from hippocampal slices
Label free bio image sensor for real time monitoring of potassium ion released from hippocampal slices
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DOI：
10.1016/j.snb.2014.04.019
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发表时间：
2014-10-01
影响因子：
8.4
通讯作者：
Sawada, Kazuaki
中科院分区：
文献类型：
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作者：
Kono, Akiteru;Sakurai, Takashi;Sawada, Kazuaki
为了评估海马片周围细胞外钾离子浓度([K+](OUT))的二维分布，我们研制了一种128×128像素型钾离子敏感图像传感器(PIS)，该传感器由电荷耦合型pH图像传感器和含有钾离子敏感离子载体伐林霉素的聚氯乙烯(PVC)塑化膜组成。当钾离子浓度在10(-3)-10(-1)M之间时，电势斜率为50 mV/ten。虽然在没有脑片或脑片位置之外的区域没有记录到信号的变化，但在海马片下检测到了显著的[K+](OUT)变化。我们成功地检测到谷氨酸诱导的与通过钾离子通道门控相关的海马神经元[K+](OUT)的变化，而不需要标记过程。在谷氨酸刺激的传感器和切片之间的区域，[K+](OUT)从10 mm增加到50 mm。(C)2014爱思唯尔B.V.保留所有权利。
To assess the two dimensional distribution of the extracellular potassium ion concentration ([K+](out)) surrounding hippocampal slices, we developed a 128 x 128 pixel type potassium ion-sensitive image sensor (PIS) composed of a charge-coupled-type pH image sensor and a poly( vinyl chloride) (PVC) plasticized membrane containing valinomycin, a potassium ion-sensitive ionophore. The potential slope was 50 mV/decade for a potassium ion concentration between 10(-3) and 10(-1)M. Although variations in the signal were not recorded in the absence of a brain slice or in areas outside of the slice position, significant changes in [K+](out) were detected underneath the hippocampal slices. We successfully detected the glutamate-induced changes in [K+](out) associated with gating through potassium ion channels in hippocampal neurons without a labeling process. [K+](out) increased from 10 mM to 50 mM in the area between the sensor and slice stimulated with glutamate. (C) 2014 Elsevier B.V. All rights reserved.