Immunolocalization of types I, II, and X collagen in the tibial insertion sites of the medial meniscus
Immunolocalization of types I, II, and X collagen in the tibial insertion sites of the medial meniscus
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DOI：
10.1007/s001670050013
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发表时间：
2000-01-01
影响因子：
3.8
通讯作者：
Gao, JZ
中科院分区：
文献类型：
--
作者：
Gao, JZ
兔膝关节的内侧半月板通过前部和后部插入附着于胫骨平台。完整的半月板胫骨插入对于半月板功能至关重要。在本研究中，通过间接免疫组织化学方法在兔模型中研究了半月板胫骨插入物中 I、II 和 X 型胶原的分布。在前插入部位通过组织学鉴定了四个组织区，包括韧带区、未钙化和钙化的纤维软骨区和骨；在后插入部位未观察到韧带区。发现韧带组织和骨骼中 I 型胶原蛋白的标记较强，而纤维软骨中的 I 型胶原蛋白标记较弱，纤维软骨也被标记为 II 型胶原蛋白。不同类型胶原呈阳性的组织重叠并形成不同角度和深度的不规则界面，特别是在钙化的纤维软骨和骨之间的界面处。仅在钙化纤维软骨区鉴定出 X 型胶原蛋白的阳性标记。半月板胫骨插入物中 I 型和 II 型胶原蛋白的共存可能表明该结构单元同时承受压缩载荷和拉伸载荷。 X型胶原蛋白可能在维持该组织区域的钙化状态方面发挥作用，使其机械刚度保持在未钙化的纤维软骨和硬骨之间。对于功能性半月板替代，应考虑恢复插入结构，包括独特的胶原分布。
The medial meniscus of the rabbit knee joint attaches to the tibial plateau via anterior and posterior insertions. Intact meniscal tibial insertions are essential for meniscal function. In the present study the distributions of types I, II, and X collagen in meniscal tibial insertions were investigated by indirect immunohistochemistry in a rabbit model. Four tissue zones were histologically identified in the anterior insertion site, including the ligamentous zone, uncalcified and calcified fibrocartilaginous zones and bone; the ligamentous zone was not observed in the posterior insertion site. Labeling for type I collagen was found to be strong in the ligament tissue and bone, and weak in the fibrocartilages which were also labeled for type II collagen. Tissues positive for different types of collagen overlapped and formed an irregular interface with various angles and depths, especially at the interface between the calcified fibrocartilage and bone. Positive labeling for type X collagen was identified only in the calcified fibrocartilage zone. The coexistence of types I and II collagen in the meniscal tibial insertions may indicate that this structural unit is subjected to both compressive and tensile loads. Type X collagen may play a role in maintaining the calcifying status of this tissue zone, so that its mechanical stiffness is kept between that of uncalcified fibrocartilage and hard bone. Restoration of the insertional structure including the distinct collagen distribution should be considered for a functional meniscal substitution.