Divergence in Diversity and Composition of Root-Associated Fungi Between Greenhouse and Field Studies in a Semiarid Grassland
Divergence in Diversity and Composition of Root-Associated Fungi Between Greenhouse and Field Studies in a Semiarid Grassland
复制标题
DOI：
10.1007/s00248-018-1277-y
复制
发表时间：
2019-07-01
影响因子：
3.6
通讯作者：
Rudgers, Jennifer A.
中科院分区：
文献类型：
--
作者：
Chung, Y. Anny;Jumpponen, A.;Rudgers, Jennifer A.
植物-土壤反馈（PSF）和植物-微生物相互作用的研究通常完全依赖于温室实验，但我们对如何以及何时可以推断结果来解释自然现象知之甚少。使用相同的宿主物种在不同的研究环境中对微生物群落进行系统的比较可以为此类实验的普遍性提供信息。我们使用Illumina MiSeq测序来表征来自温室PSF实验、田间PSF实验、田间单作林和田间自然存在的常驻植物的两种基础草的根相关真菌。一个核心社区的总序列丰度的50%发生在所有研究类型的植物，证明了田间和温室实验的能力，以捕捉自然群落的主导成分。在所有研究类型中，真菌群落都具有植物物种特异性，核心群落比外围类群表现出更强的宿主特异性。从温室和现场PSF实验的根有较低的样本之间的变化，在社区组成和较高的多样性比那些从自然发生的，或种植的单作植物从外地。核心和总的真菌组成差异很大，在不同的研究类型，真菌群落之间的差异并没有预测植物土壤反馈实验中测量。这些结果表明，根瘤菌组装机制在自然界中不同的短期，接种研究的动态。我们的研究结果验证了常见的PSF实验设计测试土壤接种效果的有效性，并强调了从全群落接种实验到自然生态系统的植物响应的潜在微生物机制的挑战。
Investigations of plant-soil feedbacks (PSF) and plant-microbe interactions often rely exclusively on greenhouse experiments, yet we have little understanding of how, and when, results can be extrapolated to explain phenomena in nature. A systematic comparison of microbial communities using the same host species across study environments can inform the generalizability of such experiments. We used Illumina MiSeq sequencing to characterize the root-associated fungi of two foundation grasses from a greenhouse PSF experiment, a field PSF experiment, field monoculture stands, and naturally occurring resident plants in the field. A core community consisting 50% of total sequence abundance occurred in plants from all study types, demonstrating the ability of field and greenhouse experiments to capture the dominant component of natural communities. Fungal communities were plant species-specific across the study types, with the core community showing stronger host specificity than peripheral taxa. Roots from the greenhouse and field PSF experiments had lower among sample variability in community composition and higher diversity than those from naturally occurring, or planted monoculture plants from the field. Core and total fungal composition differed substantially across study types, and dissimilarity between fungal communities did not predict plant-soil feedbacks measured in experiments. These results suggest that rhizobiome assembly mechanisms in nature differ from the dynamics of short-term, inoculation studies. Our results validate the efficacy of common PSF experiment designs to test soil inoculum effects, and highlight the challenges of scaling the underlying microbial mechanisms of plant responses from whole-community inoculation experiments to natural ecosystems.