Biomimetic Surface-Enhanced Raman Scattering Nanoparticles with Improved Dispersibility, Signal Brightness, and Tumor Targeting Functions
Biomimetic Surface-Enhanced Raman Scattering Nanoparticles with Improved Dispersibility, Signal Brightness, and Tumor Targeting Functions
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DOI：
10.1021/acsnano.2c01062
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发表时间：
2022-05-24
期刊：
影响因子：
17.1
通讯作者：
Nie, Shuming
中科院分区：
文献类型：
--
作者：
Srivastava, Indrajit;Xue, Ruiyang;Nie, Shuming
生物相容性和无毒的表面增强拉曼散射（SERS）纳米粒子的发展是相当大的当前兴趣，因为它们有吸引力的生物医学应用，如超灵敏的体外诊断，体内肿瘤成像，和光谱引导的癌症手术。然而，目前的SERS纳米颗粒是在水溶液中制备和储存的，具有有限的稳定性和可重复性，并且不适合通过冷冻干燥或其他方式进行冻干和储存。在这里，我们报告了一个简单但强大的方法来涂覆胶体SERS纳米粒子的天然衍生的仿生红细胞膜（RBCM），导致显着改善的稳定性和在冻融，冻干，加热和生理条件下的稳定性。结果表明，冻干的固体形式的SERS纳米颗粒可以容易地溶解和分散在生理缓冲溶液中。一个令人惊讶的发现是，在类似的实验条件下，RBCM涂覆的SERS颗粒比PEG化的SERS颗粒亮得多（多达5倍）。这种额外的增强被认为是由RBCM的烃链的疏水性质引起的，已知其减少电子阻尼并提高电磁场增强。使用仿生膜涂层的另一个优点是双层膜结构允许非价插入分子配体用于肿瘤靶向。特别是，我们表明，环-RGD，肿瘤靶向肽，可以有效地插入到膜涂层的SERS纳米粒子靶向的α nu β 3整合素受体上表达的癌细胞。因此，与传统聚乙二醇相比，仿生RBCM在制备SERS纳米颗粒方面具有重大优势，具有更好的分散性、更高的信号强度和更有效的生物功能化。
The development of biocompatible and nontoxic surface-enhanced Raman scattering (SERS) nanoparticles is of considerable current interest because of their attractive biomedical applications such as ultrasensitive in vitro diagnostics, in vivo tumor imaging, and spectroscopy-guided cancer surgery. However, current SERS nanoparticles are prepared and stored in aqueous solution, have limited stability and dispersibility, and are not suitable for lyophilization and storage by freeze-drying or other means. Here, we report a simple but robust method to coat colloidal SERS nanoparticles by naturally derived biomimetic red blood cell membranes (RBCM), leading to a dramatic improvement in stability and dispersibility under freeze-thawing, lyophilization, heating, and physiological conditions. The results demonstrate that the lyophilized SERS nanoparticles in the solid form can be readily dissolved and dispersed in physiological buffer solutions. A surprising finding is that the RBCM-coated SERS particles are considerably brighter (by as much as 5-fold) than PEGylated SERS particles under similar experimental conditions. This additional enhancement is believed to arise from the hydrophobic nature of RBCM's hydrocarbon chains, which is known to reduce electronic dampening and boost electromagnetic field enhancement. A further advantage in using biomimetic membrane coatings is that the bilayer membrane structure allows nonvalent insertion of molecular ligands for tumor targeting. In particular, we show that cyclic-RGD, a tumor-targeting peptide, can be efficiently inserted into the membrane coatings of SERS nanoparticles for targeting the a nu beta 3 integrin receptors expressed on cancer cells. Thus, biomimetic RBCMs provide major advantages over traditional polyethylene glycols for preparing SERS nanoparticles with improved dispersibility, higher signal intensity, and more efficient biofunctionalization.