The Effect of Novel Fluorapatite Surfaces on Osteoblast-Like Cell Adhesion, Growth, and Mineralization
The Effect of Novel Fluorapatite Surfaces on Osteoblast-Like Cell Adhesion, Growth, and Mineralization
复制标题
DOI：
10.1089/ten.tea.2009.0632
复制
发表时间：
2010-09-01
影响因子：
4.1
通讯作者：
Clarkson, Brian H.
中科院分区：
文献类型：
--
作者：
Liu, Jun;Jin, Taocong;Clarkson, Brian H.
对生物医学植入物的需求不断增加，以纠正由创伤、疾病或遗传疾病引起的骨骼缺陷。在本研究中，MG-63细胞生长在有序和无序的氟磷灰石(FA)晶体表面的金属上，以研究其生物相容性、初始细胞反应以及在此过程中的潜在机制。并对细胞的长期生长和矿化情况进行了研究。3天后，酸蚀金属表面的细胞数明显高于有序和无序FA表面的细胞数，但大量细胞的初始黏附并没有导致细胞-种植体界面更早的矿物形成。在84个细胞黏附和基质聚焦途径基因中，共有15个基因表达上调或下调，如整合素分子、整合素αM和整合素α7和8，这表明与无序的表面相比，有序的FA表面对这些黏附分子具有调控作用。成骨诱导(OI)7周后，FA表面骨钙素表达和矿化结节形成最为明显。无论有无OI，有序FA表面与金属的结合力均显著高于无序FA表面。最重要的是，即使没有补充OI，生长在FA晶体表面的MG-63细胞也开始分化和矿化，这表明FA晶体可能是一种简单的生物活性种植涂层材料。
There is increasing demand for biomedical implants to correct skeletal defects caused by trauma, disease, or genetic disorder. In this study, the MG-63 cells were grown on metals coated with ordered and disordered fluorapatite (FA) crystal surfaces to study the biocompatibility, initial cellular response, and the underlying mechanisms during this process. The long-term growth and mineralization of the cells were also investigated. After 3 days, the cell numbers on etched metal surface are significantly higher than those on the ordered and disordered FA surfaces, but the initial adherence of a greater number of cells did not lead to earlier mineral formation at the cell-implant interface. Of the 84 cell adhesion and matrix-focused pathway genes, an up-or down-regulation of a total of 15 genes such as integrin molecules, integrin alpha M and integrin alpha 7 and 8 was noted, suggesting a modulating effect on these adhesion molecules by the ordered FA surface compared with the disordered. Osteocalcin expression and the mineral nodule formation are most evident on the FA surfaces after osteogenic induction (OI) for 7 weeks. The binding of the ordered FA surfaces to the metal, with and without OI, was significantly higher than that of the disordered FA surfaces with OI. Most significantly, even without the OI supplement, the MG-63 cells grown on FA crystal surfaces start to differentiate and mineralize, suggesting that the FA crystal could be a simple and bioactive implant coating material.