Regucalcin enhances adipocyte differentiation and attenuates inflammation in 3T3-L1 cells.
Regucalcin enhances adipocyte differentiation and attenuates inflammation in 3T3-L1 cells.
复制标题
DOI：
10.1002/2211-5463.12947
复制
发表时间：
2020-10
期刊：
影响因子：
2.6
通讯作者：
Kaneda N
中科院分区：
文献类型：
--
作者：
Murata T;Yamaguchi M;Kohno S;Takahashi C;Risa W;Hatori K;Hikita K;Kaneda N
脂肪细胞分化的失调和功能障碍在肥胖和相关疾病如糖尿病和代谢综合征的发病机制中起关键作用，因此，更好地理解脂肪形成的分子机制可能有助于阐明肥胖及其相关疾病的病理状况。Regucalcin（RGN）在哺乳动物细胞内Ca ~（2+）信号通路中起着多种调节作用。在这里，我们报告了RGN的过表达增强了脂肪形成刺激后3 T3-L1脂肪细胞中的脂质蓄积，并伴随着脂肪细胞分化标志物蛋白的上调。相反，RGN的遗传破坏抑制了3 T3-L1细胞的脂肪形成刺激诱导的分化。此外，在transwell共培养系统中，分化的3 T3-L1脂肪细胞中的RGN过表达阻断了3 T3-L1脂肪细胞和RAW264.7巨噬细胞之间的炎症串扰。在共培养的3 T3 ‐L1脂肪细胞中RGN表达的敲低增强了它们对RAW 264.7巨噬细胞介导的炎症的易感性。这些结果表明，RGN是3 T3-L1脂肪细胞分化所必需的，并且在与RAW 264.7巨噬细胞共培养后，它对3 T3-L1脂肪细胞炎症具有抗炎活性。因此，RGN可能是脂肪细胞分化的一种新型调节剂，并在巨噬细胞浸润的脂肪细胞组织中起到炎症抑制剂的作用。Regucalcin促进3 T3-L1细胞分化为脂肪细胞。在transwell共培养系统中，3 T3-L1脂肪细胞中的Regucalcin还阻断3 T3-L1脂肪细胞和RAW264.7巨噬细胞之间的炎性串扰。Regucalcin是脂肪细胞分化的新型调节剂，并作为巨噬细胞相关脂肪细胞炎症的抑制剂。
Dysregulation of adipocyte differentiation and dysfunction play key roles in the pathogenesis of obesity and associated disorders such as diabetes and metabolic syndrome, and as such, a better understanding of the molecular mechanism of adipogenesis may help to elucidate the pathological condition of obesity and its associated disorders. Regucalcin (RGN) plays multiple regulatory roles in intracellular Ca2+ signaling pathways in mammalian cells. Here, we report that overexpression of RGN enhances lipid accumulation in 3T3‐L1 adipocyte cells after adipogenic stimulation, accompanied by upregulation of adipocyte differentiation marker proteins. In contrast, genetic disruption of RGN inhibited adipogenic stimulation‐induced differentiation of 3T3‐L1 cells. Furthermore, RGN overexpression in differentiated 3T3‐L1 adipocytes blocked inflammatory crosstalk between 3T3‐L1 adipocytes and RAW264.7 macrophages in a transwell coculture system. Knockdown of RGN expression in cocultured 3T3‐L1 adipocytes enhanced their susceptibility to RAW264.7 macrophage‐mediated inflammation. These results suggest that RGN is required for 3T3‐L1 adipocyte differentiation and that it exerts anti‐inflammatory activity against 3T3‐L1 adipocyte inflammation after coculture with RAW264.7 macrophages. Thus, RGN may be a novel regulator of adipocyte differentiation and act as a suppressor of inflammation in macrophage‐infiltrated adipocyte tissue. Regucalcin enhances the differentiation of 3T3‐L1 cells into adipocytes. Regucalcin in 3T3‐L1 adipocytes also blocks inflammatory crosstalk between 3T3‐L1 adipocytes and RAW264.7 macrophages in a transwell coculture system. Regucalcin is a novel regulator of adipocyte differentiation and acts as a suppressor of macrophage‐associated adipocyte inflammation.
登录
查看更多内容
影响因子：
3.2
作者：
Okada H;Senmaru T;Fukui M;Kondo Y;Ishigami A;Maruyama N;Obayashi H;Yamazaki M;Nakamura N;Hasegawa G
通讯作者：
Hasegawa G
影响因子：
2.2
作者：
Hasegawa, Goji
通讯作者：
Hasegawa, Goji
影响因子：
10.8
作者：
Guzik TJ;Skiba DS;Touyz RM;Harrison DG
通讯作者：
Harrison DG
影响因子：
2.6
作者：
Murata T;Yamaguchi M;Kohno S;Takahashi C;Kakimoto M;Sugimura Y;Kamihara M;Hikita K;Kaneda N
通讯作者：
Kaneda N
影响因子：
6.5
作者：
Fu, YC;Luo, NL;Garvey, WT
通讯作者：
Garvey, WT