Single-Cell MALDI-MS as an Analytical Tool for Studying Intrapopulation Metabolic Heterogeneity of Unicellular Organisms
Single-Cell MALDI-MS as an Analytical Tool for Studying Intrapopulation Metabolic Heterogeneity of Unicellular Organisms
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DOI：
10.1021/ac1015326
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发表时间：
2010-09-01
影响因子：
7.4
通讯作者：
Zenobi, Renato
中科院分区：
文献类型：
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作者：
Amantonico, Andrea;Urban, Pawel L.;Zenobi, Renato
异质性是所有生物种群的一个特征。在这里，我们试图验证单细胞质谱法检测单细胞生物群体中代谢物水平变化的方法。利用负模基质辅助激光解吸/电离（MALDI）质谱法（MS）可以很容易地检测到梭状芽孢杆菌单细胞中参与中枢代谢的代谢物（ADP、ATP、GTP和UDP-Glucose）。用标准化合物对该方法的分析能力进行了表征。该方法随后被用于研究具有不同水平所选代谢物的单个细胞群。利用主成分分析和支持向量机算法，可以实现不同代谢状态下棘球蚴个体细胞的清晰分离。本研究证明了单细胞代谢物质谱分析在测量细胞群体异质性方面的适用性。
Heterogeneity is a characteristic feature of all populations of living organisms. Here we make an attempt to validate a single-cell mass spectrometric method for detection of changes in metabolite levels occurring in populations of unicellular organisms. Selected metabolites involved in central metabolism (ADP, ATP, GTP, and UDP-Glucose) could readily be detected in single cells of Closterium acerosum by means of negative-mode matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). The analytical capabilities of this approach were characterized using standard compounds. The method was then used to study populations of individual cells with different levels of the chosen metabolites. With principal component analysis and support vector machine algorithms, it was possible to achieve a clear separation of individual C. acerosum cells in different metabolic states. This study demonstrates the suitability of mass spectrometric analysis of metabolites in single cells to measure cell-population heterogeneity.